Non-permeable cryoprotectants' influence on fibroblast slow freezing in six-banded armadillo.

Background: There is a crucial need to develop appropriate cryopreservation solutions so that somatic resource biobanks of wildlife can be established.

Objective: Here, we propose a cryopreservation protocol to optimize the preservation of skin-derived fibroblasts from six-banded armadillos (Euphractus sexcinctus Linnaeus, 1758) by comparing different concentrations of fetal bovine serum (FBS) in the absence or presence of sucrose as non-permeable cryoprotectants.

Materials And Methods: Cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium (DMEM) with 10% dimethyl sulfoxide (DMSO), varying concentrations of FBS (10, 20 and 40%) without or with 0.

Multimodal management of a persistent labial hemangioma: a case report.

Hemangiomas are benign vascular tumors that are relatively unusual in the oral cavity. The management of labial hemangiomas may be challenging for functional and esthetic reasons. In the present case, a 12-year-old boy with a large congenital hemangioma in the area of the upper lip was referred due to esthetic concerns and a history of treatment failure.

Sleep quality and duration and frailty in older adults: a systematic review.

Introduction: Sleep problems and frailty are associated with adverse health outcomes in older adults, including mortality, and constitute a major public health challenge.

Objective: This study investigated the association between sleep quality and duration and frailty in older adults, with emphasis on methods of evaluation.

Methods: This systematic review was guided by the Preferred Reporting Items for Systematic Reviews and MetaAnalyses (PRISMA).

Geographical origin authentication of instant coffee from southern Bahia using MIR and NIR spectroscopy coupled with DD-SIMCA.

This study presents an innovative approach for authenticating the geographical origin of instant coffee from Southern Bahia using vibrational spectral data coupled with Data-Driven Soft Independent Modeling of Class Analogy (DD-SIMCA). Combining data from a benchtop mid-infrared (MIR) spectrometer and a portable near-infrared (NIR) spectrometer, the methodology achieved 100 % efficiency with Multiplicative Scatter Correction (MSC) and Standard Normal Variate (SNV) pre-processing, alongside offset correction for MIR data. MIR spectroscopy enabled detailed chemical composition analysis, while the portable NIR spectrometer provided cost-effective in situ capabilities.

British Society of Gastroenterology practice guidance on the management of acute and chronic gastrointestinal symptoms and complications as a result of treatment for cancer.

Background: Survival rates after a diagnosis of cancer are improving. Poorly managed gastrointestinal (GI) side effects can interfere with delivery of curative cancer treatment. Long-term physical side effects of cancer therapy impinge on quality of life in up to 25% of those treated for cancer, and GI side effects are the most common and troublesome.