Publications by authors named "D Ouwerkerk"

poisoning of cattle causes distinct symptoms and frequently death, attributable to the toxin simplexin. poisoning was induced via addition of ground plant to the daily feed in a three-month trial with Droughtmaster steers. The trial tested four potential mitigation treatments, namely, biochar, activated biochar, bentonite, and a bacterial inoculum, and incorporated negative and positive control groups.

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poisoning of cattle is toxicologically linked to the activation of bovine protein kinase C (PKC) by the plant-derived toxin simplexin. To understand the affinity of PKC for simplexin, we performed molecular dynamics (MD) studies of simplexin, simplexin analogues, and several other activators of PKC. Binding enthalpy calculations indicated that simplexin had the strongest affinity for PKCα-C1B among the activators studied.

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We report the 2.78-Mb circular genome sequence of sp. strain YE332, isolated from a fermentation of bovine rumen fluid, supplied with leaf material from cv.

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Article Synopsis
  • Pimelea poisoning is caused by the toxic compound simplexin found in native Australian Pimelea plants, affecting cattle.
  • Researchers aimed to understand how rumen microorganisms metabolize simplexin by analyzing its breakdown products through various acid hydrolysis and advanced mass spectrometry techniques.
  • Despite successfully identifying potential simplexin metabolites in lab conditions, the study found that these metabolites could not be detected in biological samples, indicating that they may not be produced by rumen microorganisms or are further metabolized.
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Aims: Sub-therapeutic use of antibiotics as a growth promoter in animal diets has either been banned or voluntarily withdrawn from use in many countries to help curb the emergence of antibiotic-resistant pathogens. Probiotics may be an alternative to antibiotics as a growth promoter. We investigated the effects of a novel probiotic strain, Bacillus amyloliquefaciens H57 (H57) on the performance and microbiome-associated metabolic potential.

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