Electrophoretic Mobility Assay to Separate Supercoiled, Catenated, and Knotted DNA Molecules.

Two-dimensional (2D) agarose gel electrophoresis is the method of choice to analyze DNA topology. The possibility to use strains with different genetic backgrounds in combination with nicking enzymes and different concentrations of norfloxacin improves the resolution of 2D gels to study the electrophoretic behavior of three different families of DNA topoisomers: supercoiled DNA molecules, post-replicative catenanes, and knotted DNA molecules. Here, we describe the materials and procedures required to optimize their separation by 2D gels.

Ultrafast Excitation Exchange in a Maxwell Fish-Eye Lens.

The strong coupling of quantum emitters to a cavity mode has been of paramount importance in the development of quantum optics. Recently, also the strong coupling to more than a single mode of an electromagnetic resonator has drawn considerable interest. We investigate how this multimode strong coupling regime can be harnessed to coherently control quantum systems.

Driven-Dissipative Time Crystalline Phases in a Two-Mode Bosonic System with Kerr Nonlinearity.

For the driven-dissipative system of two coupled bosonic modes in a nonlinear cavity resonator, we demonstrate a sequence of phase transitions from a trivial steady state to two distinct dissipative time crystalline phases. These effects are already anticipated at the level of the semiclassical analysis of the Lindblad equation using the theory of bifurcations and are further supported by the full quantum treatment. The system is predicted to exhibit different dynamical phases characterized by an oscillating nonequilibrium steady state with nontrivial periodicity, which is a hallmark of time crystals.

Two-Dimensional Gel Electrophoresis to Study the Activity of Type IIA Topoisomerases on Plasmid Replication Intermediates.

DNA topoisomerases are the enzymes that regulate DNA topology in all living cells. Since the discovery and purification of ω (omega), when the first were topoisomerase identified, the function of many topoisomerases has been examined. However, their ability to relax supercoiling and unlink the pre-catenanes of partially replicated molecules has received little attention.

Periodic Cavity State Revivals from Atomic Frequency Combs.

Spin ensembles with a comb-shaped spectrum have shown exciting properties as efficient quantum memories. Here, we present a rigorous theoretical study of such atomic frequency combs in the strong coupling limit of cavity QED, based on a full quantum treatment using tensor-network methods. Our results demonstrate that arbitrary multiphoton states in the cavity are almost perfectly absorbed by the spin ensemble and reemitted as parity-flipped states at periodic time intervals.