Publications by authors named "D Nowis"
Multiple myeloma (MM), one of the most frequent haematological malignancies, significantly increases the risk of bacterial infections due to treatment-related side effects, comorbidities and cancer-induced immune deficiencies. Recently, CD71 erythroid cells (CECs) have been identified as key immunomodulators in neonates and cancer patients, but their role in MM progression remains unclear. Using a murine MM model, closely resembling human disease, we observed that MM progression is associated with anaemia and an increase in immature CECs, which are characterized by elevated arginase 2 (ARG2) expression.
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- Tumoroscope is a new probabilistic model that accurately identifies cancer clones and their locations in tumors using pathological images, whole exome sequencing, and spatial transcriptomics data, achieving near single-cell resolution.
- Unlike previous methods, Tumoroscope effectively resolves the proportions of different cancer clones within spatial transcriptomics spots.
- The model has been applied to prostate and breast cancer datasets, revealing spatial patterns of clone distribution and allowing the analysis of clone-specific gene expression.
This work presents a comprehensive evaluation of the role of p66Shc protein in mitochondrial physiology in MDA-MB-231 breast cancer cells. The use of human breast cancer cell line MDA-MB-231 and its genetically modified clones (obtained with the use of the CRISPR-Cas9 technique), expressing different levels of p66Shc protein, allowed us to demonstrate how the p66Shc protein affects mitochondrial metabolism of human breast cancer cells. Changes in the level of p66Shc (its overexpression, and overexpressing of its Serine 36-mutated version, as well as the knockout of p66Shc) exert different effects in breast cancer cells.
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- - The study investigates the potential of chemically modified in vitro transcribed (IVT) mRNAs to enhance their stability and efficiency for applications like gene therapy and cancer treatment, especially focusing on the significance of the mRNA 5'-cap structure in regulating translation.
- - Researchers developed 16 new cap analogs by combining a trinucleotide IVT capping technology with various modifications to the 5' cap and added epigenetic marks that are similar to those found in the mRNA of more complex organisms.
- - The effects of these new analogs were tested on their interaction with a key protein involved in translation, as well as their translation efficiency in cell models, with one promising modification further evaluated in a mouse model to assess
Inflammasome assembly is a potent mechanism responsible for the host protection against pathogens, including viruses. When compromised, it can allow viral replication, while when disrupted, it can perpetuate pathological responses by IL-1 signaling and pyroptotic cell death. SARS-CoV-2 infection was shown to activate inflammasome in the lungs of COVID-19 patients, however, potential mechanisms responsible for this response are not fully elucidated.
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