Spectral studies of the interaction between amanitine and ethidium bromide fluorophore showed the appearance of a new intensive fluorescence band after addition of amanitine to ethidium bromide solution, caused by the formation of a charge-transfer complex. The new fluorescence band is located in a shorter wave region of the spectrum compared to ethidium bromide fluorescence band. Based on the results, a rapid fluorescent method for detection of amanitines was developed.
View Article and Find Full Text PDFThe peripheral binding site of horse serum cholinesterase (EC 3.1.1.
View Article and Find Full Text PDFBull Exp Biol Med
February 2005
We present the results of spectral studies of the interactions between butyrylcholinesterase and ethidium bromide fluorophore inhibitor. The ethidium bromide fluorescence selectively increased in the presence of butyrylcholinesterase. A rapid method for evaluation of serum butyrylcholinesterase activity is developed.
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