Publications by authors named "D N Shtokalo"
Introduction: Modification of natural enzymes to introduce new properties and enhance existing ones is a central challenge in bioengineering. This study is focused on the development of Taq polymerase mutants that show enhanced reverse transcriptase (RTase) activity while retaining other desirable properties such as fidelity, 5'- 3' exonuclease activity, effective deoxyuracyl incorporation, and tolerance to locked nucleic acid (LNA)-containing substrates. Our objective was to use AI-driven rational design combined with multiparametric wet-lab analysis to identify and validate Taq polymerase mutants with an optimal combination of these properties.
View Article and Find Full Text PDFTyrosyl-DNA phosphodiesterase 1 (TDP1) is a human DNA repair protein. It is a member of the phospholipase D family based on structural similarity. TDP1 is a key enzyme of the repair of stalled topoisomerase 1 (TOP1)-DNA complexes.
View Article and Find Full Text PDFBackground: Cardiovascular disease had a global prevalence of 523 million cases and 18.6 million deaths in 2019. The current standard for diagnosing coronary artery disease (CAD) is coronary angiography either by invasive catheterization (ICA) or computed tomography (CTA).
View Article and Find Full Text PDFArticle Synopsis
- Topoisomerase 1 (TOP1) is crucial for DNA functions like replication and is targeted by anticancer drugs such as topotecan, which can cause cell death by stabilizing the TOP1 cleavage complex.
- Tyrosyl-DNA phosphodiesterase 1 (TDP1) can remove this complex, thereby reducing the effectiveness of topotecan.
- A study comparing wild type and PARP1 knockout HEK293A cells revealed that PARP1 deficiency led to significantly more changes in gene expression when treated with topotecan and a TDP1 inhibitor, affecting pathways related to cancer development and DNA repair.
Background: Despite proven therapeutic effects in inflammatory conditions, the specific mechanisms of phytochemical therapies are not well understood. The transcriptome effects of Traumeel (Tr14), a multicomponent natural product, and diclofenac, a non-selective cyclooxygenase (COX) inhibitor, were compared in a mouse cutaneous wound healing model to identify both known and novel pathways for the anti-inflammatory effect of plant-derived natural products.
Methods: Skin samples from abraded mice were analyzed by single-molecule, amplification-free RNAseq transcript profiling at 7 points between 12 and 192 h after injury.