Publications by authors named "D N Shchepkin"

The cardiac myosin binding protein-C (cMyBP-C) regulates cross-bridge formation and controls the duration of systole and diastole at the whole heart level. As known, mutations in cMyBP-C increase the cross-bridge number and rate of their cycling, hypercontractility, and myocardial hypertrophy. We investigated the effects of the mutations D75N and P161S of cMyBP-C related to hypertrophic cardiomyopathy on the mechanism of force generation in isolated slow skeletal muscle fibers.

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About half of the mutations that lead to hypertrophic cardiomyopathy (HCM) occur in the gene. However, the molecular mechanisms of pathogenicity of point mutations in cardiac myosin-binding protein C (cMyBP-C) remain poorly understood. In this study, we examined the effects of the D75N and P161S substitutions in the C0 and C1 domains of cMyBP-C on the structural and functional properties of the C0-C1-m-C2 fragment (C0-C2).

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Article Synopsis
  • The study addresses the antibiotic resistance crisis by proposing new monitoring protocols and emphasizing the need for innovative antibiotics, highlighting challenges like diverse resistance factors and limitations in current drug discovery methods.
  • Researchers analyzed metagenomes from 658 topsoil samples across Europe, focusing on genes associated with antibiotic production and resistance, revealing distinctive patterns in gene distribution linked to environmental factors.
  • Findings indicate that agricultural practices lead to a homogenization of microbial antibiotic systems, underscoring the value of preserving diverse ecosystems, while providing principles to enhance antibiotic discovery and understand resistance gene dispersion.
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The actin cytoskeleton is one of the most important players in cell motility, adhesion, division, and functioning. The regulation of specific microfilament formation largely determines cellular functions. The main actin-binding protein in animal cells is tropomyosin (Tpm).

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