Enhanced Oil Recovery Method Selection for Shale Oil Based on Numerical Simulations.

As unconventional reserves, oil shale deposits require additional oil recovery techniques to achieve favorable production levels. The efficiency of a shale reservoir development project is highly dependent on the application of enhanced oil recovery (EOR) techniques. There are many studies devoted to discrete investigations of each EOR method.

Effect of Arginine on Chaperone-Like Activity of HspB6 and Monomeric 14-3-3ζ.

The effect of protein chaperones HspB6 and the monomeric form of the protein 14-3-3ζ (14-3-3ζ) on a test system based on thermal aggregation of UV-irradiated glycogen phosphorylase (UV-Ph) at 37 °C and a constant ionic strength (0.15 M) was studied using dynamic light scattering. A significant increase in the anti-aggregation activity of HspB6 and 14-3-3ζ was demonstrated in the presence of 0.

Concatenation of 14-3-3 with partner phosphoproteins as a tool to study their interaction.

Regulatory 14-3-3 proteins interact with a plethora of phosphorylated partner proteins, however 14-3-3 complexes feature intrinsically disordered regions and often a transient type of interactions making structural studies difficult. Here we engineer and examine a chimera of human 14-3-3 tethered to a nearly complete partner HSPB6 which is phosphorylated by protein kinase A (PKA). HSPB6 includes a long disordered N-terminal domain (NTD), a phosphorylation motif around Ser16, and a core α-crystallin domain (ACD) responsible for dimerisation.