and abnormalities in childhood hematological malignancies other than B-cell Acute Lymphoblastic Leukemia.

Rearrangements and overexpression of are hallmarks of poor outcomes in -like B-ALL, and overexpression is a high-risk marker in T-ALL. However, alterations in pediatric hematologic malignancies other than B-ALL have not been reported. In this study, we analyzed the overexpression, rearrangements ( and ), activation (pSTAT5 and pERK), and the expression of dominant-negative isoforms (Ik6 and Ik8), implied in dysregulation, in 16 pediatric patients (AML,  = 9; T-ALL,  = 3; LBL,  = 2; HL,  = 1; cytopenia,  = 1).

Exposure to Insecticides Modifies Gene Expression and DNA Methylation in Hematopoietic Tissues In Vitro.

The evidence supporting the biological plausibility of the association of permethrin and malathion with hematological cancer is limited and contradictory; thus, further studies are needed. This study aimed to investigate whether in vitro exposure to 0.1 μM permethrin and malathion at 0, 24, 48 and 72 h after cell culture initiation induced changes in the gene expression and DNA methylation in mononuclear cells from bone marrow and peripheral blood (BMMCs, PBMCs).

Self-regulation of TNF-α Induces Dysfunction of Endothelial Colony-forming Cells from Patients with Venous Thromboembolic Disease.

Background: Endothelial colony-forming cells (ECFCs) contribute to postnatal vasculogenesis. In venous thromboembolic disease (VTD), they are functionally abnormal and produce high concentrations of TNF-α.

Objective: To analyze the TNF-α signaling pathway and its relationship with the expression of cell-cycle regulators.

Cell Contact with Endothelial Cells Favors the In Vitro Maintenance of Human Chronic Myeloid Leukemia Stem and Progenitor Cells.

Chronic Myeloid Leukemia (CML) originates in a leukemic stem cell that resides in the bone marrow microenvironment, where they coexist with cellular and non-cellular elements. The vascular microenvironment has been identified as an important element in CML development since an increase in the vascularization has been suggested to be related with poor prognosis; also, using murine models, it has been reported that bone marrow endothelium can regulate the quiescence and proliferation of leukemic stem and progenitor cells. This observation, however, has not been evaluated in primary human cells.

Apoptotic and Cell Cycle Effects of Triterpenes Isolated from on Leukemia Cell Lines.

Current antineoplastic agents present multiple disadvantages, driving an ongoing search for new and better compounds. Four lupane-type triterpenes, 3α,24-dihydroxylup-20(29)-en-28-oic acid (), 3α,23-dihydroxy-30-oxo-lup-20(29)-en-28-oic acid (), 3α,23--isopropylidenyl-3α,23-dihydroxylup-20(29)-en-28-oic acid (), and 3α,23-dihydroxylup-20(29)-en-28-oic acid (), previously isolated from , were evaluated on two cell lines of chronic (K562) and acute (HL60) myeloid leukemia. Compounds , , and decreased cell viability and inhibit proliferation, mainly in K562, and exhibited an apoptotic effect from 24 h of treatment.