Quantitative IgG response to SARS-CoV-2 membrane protein in infected individuals strongly correlates with lung injury.

The clinical manifestations of SARS-CoV-2 infection may range from asymptomatic or minor conditions to severe and life-threatening outcomes. The respiratory system is a principal target of the virus and in the majority of cases of severe disease, an acute form of pneumonia develops. Despite concerted global efforts to elucidate the pathogenic mechanisms of COVID-19, the progression of the infection leading to pulmonary damage remains poorly understood.

Quantification of Phthalate Esters in Carbonated Soft Drinks by Hollow Fiber-Microporous Membrane Liquid-Liquid Extraction and 96-Well Plate System Using Natural Deep Eutectic Solvents Based on Fatty Acids.

This study introduces a green approach to sample preparation by applying natural deep eutectic solvents (NADES) to determine phthalates in carbonated soft drinks using high-performance liquid chromatography with diode array detector (HPLC-DAD). The method employs hollow fiber-microporous membrane liquid-liquid microextraction combined with a 96-well plate system, utilizing fatty-acid-based DES in the pores of the membranes. This methodology substantially reduces the use of organic solvents, and its efficiency is comparable to or better than conventional methods.

Oncogenic PIK3CA corrupts growth factor signaling specificity.

Technical limitations have prevented understanding of how growth factor signals are encoded in distinct activity patterns of the phosphoinositide 3-kinase (PI3K)/AKT pathway, and how this is altered by oncogenic pathway mutations. We introduce a kinetic, single-cell framework for precise calculations of PI3K-specific information transfer for different growth factors. This features live-cell imaging of PI3K/AKT activity reporters and multiplexed CyTOF measurements of PI3K/AKT and RAS/ERK signaling markers over time.

The efficiency and use of a reciprocating system aid for standing and walking in children affected by severe cerebral palsy.

Cerebral Palsy (CP) is a leading cause of childhood motor disability, making independent walking a crucial therapeutic goal. Robotic assistive devices offer potential to enhance mobility, promoting community engagement and quality of life. This is an observational report of 22 cases of children with CP in which we evaluated the Moonwalker exoskeleton (a dynamic moving aid system) usability, functional changes, and caregivers' perspectives based on the International Classification of Functioning (ICF).

Early downmodulation of tumor glycolysis predicts response to fasting-mimicking diet in triple-negative breast cancer patients.

In preclinical experiments, cyclic fasting-mimicking diets (FMDs) showed broad anticancer effects in combination with chemotherapy. Among different tumor types, triple-negative breast cancer (TNBC) is exquisitely sensitive to FMD. However, the antitumor activity and efficacy of cyclic FMD in TNBC patients remain unclear.