Polar Similars: Using Massive Mobile Dating Data to Predict Synchronization and Similarity in Dating Preferences.

Leveraging a massive dataset of over 421 million potential matches between single users on a leading mobile dating application, we were able to identify numerous characteristics of effective matching. Effective matching is defined as the exchange of contact information with the likely intent to meet in person. The characteristics of effective match include alignment of psychological traits (i.

Pharmaceutical significance of the cyclic imide form of recombinant human glial cell line derived neurotrophic factor.

Purpose: The purpose of this paper is to determine the significance of cyclic imide formation of an aspartic acid residue during storage on the pharmaceutical quality of a recombinant human glial cell line-derived neurotrophic factor (rhGDNF) formulation.

Methods: A combination of chromatography, peptide mapping, mass spectroscopy, and protein sequencing was used to purify and characterize the degradation product. Circular dichroism, 1,8-ANS and heparin binding, melting temperature determination, bioassays, and preclinical pharmacokinetic and toxicology testing were performed to examine its equivalence to native rhGDNF.

Identification of Asp95 as the site of succinimide formation in recombinant human glial cell line-derived neurotrophic factor.

Human glial cell line-derived neurotrophic factor is a single polypeptide of 134 amino acids and functions as a disulfide-linked dimer. Incubation of the protein in pH 5.0 and at 37 degreesC for 1 week showed that 5% of the material was converted to a form that eluted after the major protein peak on a cation-exchange column.