Publications by authors named "D Malide"
Lysyl oxidase (LOX) is a copper-binding enzyme that cross-links elastin and collagen. The dominant LOX variation contributes to familial thoracic aortic aneurysm. Previously reported murine Lox mutants had a mild phenotype and did not dilate without drug-induced provocation.
View Article and Find Full Text PDFElastin (ELN) insufficiency leads to the cardiovascular hallmarks of the contiguous gene deletion disorder, Williams-Beuren syndrome, including hypertension and vascular stiffness. Previous studies showed that Williams-Beuren syndrome deletions, which extended to include the gene, were associated with lower blood pressure (BP) and reduced vascular stiffness. encodes for p47phox, the regulatory component of the NOX1 NADPH oxidase complex that generates reactive oxygen species (ROS) in the vascular wall.
View Article and Find Full Text PDFArticle Synopsis
- Acetyl-CoA is crucial for various cellular functions, such as ATP production, lipid synthesis, and protein modification, but how cells adapt to changes in its levels is not well understood.
- Researchers developed a cell model that uses CRISPR to adjust acetyl-CoA levels and found that reducing acetyl-CoA disrupts nucleolus function and ribosomal RNA synthesis, leading to activation of p53.
- The study suggests that histone deacetylases play a key role in this process, emphasizing the significance of acetylation in connecting acetyl-CoA changes to cellular stress responses.
Background: Ex vivo production of hematopoietic stem/precursor cells (HSPCs) represents a promising versatile approach for blood disorders.
Methods: To derive definitive HSPCs from human embryonic stem cells (ESCs), we differentiated mesodermally specified embryoid bodies (EBs) on gelatin-coated plates in serum/feeder-free conditions.
Results: Seven-day EB maturation followed by an 8-day differentiation period on OP9 cells provided the highest number of definitive (CD34+ CD235a-, 69%, p < 0.