Carvacrol (CHO), an efficient phenolic antioxidant substance for several cell types, may become a useful antioxidant for female germ cells and embryo culture. This study investigates the effects of carvacrol supplementation on bovine oocytes in maturation (IVM) and embryo production. In total, 1222 cumulus-oocyte complexes were cultured in TCM-199 alone (control treatment) or supplemented with carvacrol at the concentrations of 3 µM (Carv-3), 12.
View Article and Find Full Text PDFThe present study aimed to use an in vitro follicle culture (IVFC) biotechnique as a tool to evaluate the influence of whole flaxseed as a feed supplementation in the diet on the in vitro development of caprine early antral follicles (EAFs) and further embryo production. In total, 18 adult goats were homogeneously allocated into two diet groups: Control and Flaxseed. EAFs from both experimental groups (300-400 µm) were isolated and cultured in vitro for 18 days.
View Article and Find Full Text PDFThe relative mRNA abundance of 10 genes associated with folliculogenesis was compared between late preantral (secondary) and early antral (tertiary) ovarian follicles in goats. In total, 100 follicles in each category were mechanically isolated. The relative transcript abundance of the mRNAs were determined by qPCR.
View Article and Find Full Text PDFThe aim of this study was to evaluate the immunolocalization for glucocorticoid receptor (NR3C1) in goat ovarian follicles and the effect of cortisol on development of preantral follicles. Goat ovarian fragments were cultured for 7 days under different cortisol concentrations (0, 1, 5 and 10 ng/ml). Before and after culture, the protein expression of NR3C1 was analyzed in ovarian tissue by immunohistochemical analysis.
View Article and Find Full Text PDFReprod Domest Anim
March 2019
This study aimed to examine the in vitro culture of secondary preantral follicles, using reused ovaries, to compare both the 2D and 3D methods of in vitro culture of preantral follicles, and the system of medium replacement. Twenty-five pairs of ovaries from mixed-breed goats were used for the experiment. Follicular puncture of antral follicles was performed for in vitro production.
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