A cell-free gene expression system for prototyping and gene expression analysis.

is an obligate anaerobic, Gram-positive bacterium that produces toxins. Despite technological progress, conducting gene expression analysis of under different conditions continues to be labor-intensive. Therefore, there is a demand for simplified tools to investigate the transcriptional and translational regulation of .

Li-Based Nanoprobes with Boosted Photoluminescence for Temperature Visualization in NIR Imaging-Guided Drug Release.

Lanthanide-doped fluoride nanocrystals have emerged as promising tools in biomedicine, yet their applications are still limited by their low luminescence efficiency. Herein, we developed highly efficient lithium-based core-shell-shell (CSS) nanoprobes (NPs) featuring a rhombic active domain and a spherical inert protective shell. By introducing Yb as an energy transfer bridge and optimizing the CSS design, a remarkable 1643-fold enhancement in visible emission and a 33-fold increase in NIR emission are achieved compared to original nanoparticles.

Conformational effects in the identification and quantification of ketohydroperoxides in the oxidation of -pentane.

Stereochemistry plays a key role in both fundamental chemical processes and the dynamics of a large set of molecular systems of importance in chemistry, medicine and biology. Predicting the chemical transformations of organic precursors in such environments requires detailed kinetic models based on laboratory data. Reactive intermediates play a critical role in constraining the models but their identification and especially their quantification remain challenging.

Self-assembled eumelanin nanoparticles enhance IFN-I activation and cilia-driven intercellular communication to defend against Tulane virus, a human norovirus surrogate.

Norovirus (NoV) infection is a leading cause of gastroenteritis and poses global health threats, with increasing incidence reported in immunocompromised individuals, which is further exacerbated by the globalization of the food industry. Eumelanin has demonstrated its potential in antiviral treatments, but its role in preventing viral infections remains underexplored. Therefore, in this study, we investigated the antiviral properties and potential mechanisms of self-assembled eumelanin nanoparticles (EmNPs) against Tulane virus (TuV), a surrogate with a similar infection mechanism to NoVs.

The association between stent design and patient exercise intensity: structural coupling effects and hemodynamic analysis.

Introduction: In-stent restenosis remains a significant challenge in coronary artery interventions. This study aims to explore the relationship between exercise intensity and stent design, focusing on the coupled response of the stent structure and hemodynamics at different exercise intensities.

Methods: A coupled balloon-stent-plaque-artery model and a fluid domain model reflecting structural deformation were developed to investigate the interaction between coronary stents and stenotic vessels, as well as their impact on hemodynamics.