Publications by authors named "D M Ataeva"
Article Synopsis
- The study investigated how sodium fluoride (NaF) affects the enzymatic functions of the Klenow enzyme (KE) from E. coli DNA polymerase I using various DNA substrates.
- NaF concentrations between 5-10 mM were found to effectively inhibit the enzyme's 3'----5' exonuclease activity but did not impact its polymerase activity, indicating a selective inhibition that is dependent on magnesium ions (Mg).
- The Klenow enzyme was capable of slightly elongating primers during its reaction with specific DNA sequences, a process that can be influenced by the presence of pyrophosphate, suggesting that contaminating pyrophosphate in DNA preparations might aid nucleotide generation for primer elongation.
A study was made of reduplication of readily identifyable chromosomes 1, 2, 8 and 9 in the culture of the peripheral blood lymphocytes of Macacus mulatta. There proved to be synchronous reduplication of the chromosomes under study at the end of stages S. Areas of late-reduplication completely corresponded to the G-segments.
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