Publications by authors named "D Loew"
Quality control procedures play a pivotal role in ensuring the reliability and consistency of data generated in mass spectrometry-based proteomics laboratories. However, the lack of standardized quality control practices across laboratories poses challenges for data comparability and reproducibility. In response, we conducted a harmonization study within proteomics laboratories of the Core for Life alliance with the aim of establishing a common quality control framework, which facilitates comprehensive quality assessment and identification of potential sources of performance drift.
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- Extracellular vesicles (EVs) show promise as new treatments for cancer and degenerative diseases, but better production methods are needed to generate them efficiently from fewer cells.
- A new millifluidic cross-slot chip design enables high-yield release of biologically active EVs from less than three million cells, maintaining the cells' physiological environment for effective monitoring.
- This method allows for the release of a large number of EVs without harming the cells, revealing crucial insights into how stress affects EV production, and produces EVs with beneficial properties for wound healing and angiogenesis.
Article Synopsis
- The research investigates how shear stress affects the release and absorption of endothelial extracellular vesicles (EVs), with findings indicating that more EVs are produced under HSS conditions than LSS.
- Proteomic analysis revealed that LSS-derived EVs are enriched with proteins that enhance their uptake by endothelial cells, which may contribute to oxidative stress in those cells, highlighting the complex role of shear stress and EVs in vascular health.
Human-specific genes are potential drivers of brain evolution. Among them, SRGAP2C has contributed to the emergence of features characterizing human cortical synapses, including their extended period of maturation. SRGAP2C inhibits its ancestral copy, the postsynaptic protein SRGAP2A, but the synaptic molecular pathways differentially regulated in humans by SRGAP2 proteins remain largely unknown.
View Article and Find Full Text PDFBackground: Decellularized extracellular matrix (dECM) has been proposed as a useful source of biomimetic materials for regenerative medicine due to its biological properties that regulate cell behaviors. The present study aimed to investigate the influence of decellularized ECM derived from dental pulp stem cells (DPSCs) on gingival fibroblast (GF) cell behaviors. Cells were isolated from dental pulp and gingival tissues.
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