Effect on viability and yield on Pleurotus pulmonarius and Ganoderma lucidum after 3 years of cryopreservation without cryoprotectants.

Due to their importance in various fields such biotechnology, medicine, food science, preserving microorganisms effectively is essential. This study investigated cryopreservation of the edible fungus Pleurotus pulmonarius and the medicinal mushroom Ganoderma lucidum, for three years at -80°C in sorghum grains without cryoprotectants. We compared viability, growth, and yields with strains maintained on agar media at 5°C.