HIV OctaScanner: A Machine Learning Approach to Unveil Proteolytic Cleavage Dynamics in HIV-1 Protease Substrates.

The rise of resistance to antiretroviral drugs due to mutations in human immunodeficiency virus-1 (HIV-1) protease is a major obstacle to effective treatment. These mutations alter the drug-binding pocket of the protease and reduce the drug efficacy by disrupting interactions with inhibitors. Traditional methods, such as biochemical assays and structural biology, are crucial for studying enzyme function but are time-consuming and labor-intensive.

3D Femtosecond Laser Beam Deflection for High-Precision Fabrication and Modulation of Individual Voxelated PCM Meta-Atoms.

Optical metasurfaces have found widespread applications in the field of optoelectronic devices. However, achieving dynamic and flexible control over metasurface functionalities, while also developing simplified fabrication methods for metasurfaces, continues to pose a significant challenge. Here, the study introduces a PCM-only metasurface that exclusively consists of voxel units crafted from different phases of phase-change materials.

Effects of minocycline on patients with acute anterior circulation ischaemic stroke undergoing intravenous thrombectomy (MIST-A): the study protocol for a multicentre, prospective, randomised, open-label, blinded-endpoint trial.

Introduction: Despite the implementation of mechanical thrombectomy, acute ischaemic stroke with large vessel occlusion (AIS-LVO) remains a significant health concern, characterised by substantial morbidity and mortality. Our trial aims to evaluate the efficacy and safety of minocycline in reducing infarct volume and improving functional outcomes in patients undergoing mechanical thrombectomy for anterior circulation AIS-LVO.

Methods And Analysis: The MIST-A trial is a prospective, randomised, open-label, blinded-endpoint trial to be conducted across 12 medical centres.

Characterizing tertiary lymphoid structures associated single-cell atlas in breast cancer patients.

The tertiary lymphoid structure (TLS) is recognized as a potential prognosis factor for breast cancer and is strongly associated with response to immunotherapy. Inducing TLS neogenesis can enhance the immunogenicity of tumors and improve the efficacy of immunotherapy. However, our understanding of TLS associated region at the single-cell level remains limited.

Analysis of mechanical properties and energy evolution mechanism of frozen calcareous clay under multi-factor interaction.

Research investigating the complex mechanical properties and energy evolution mechanisms of frozen calcareous clay under the influence of multiple factors is crucial for optimizing the artificial ground freezing method in shaft sinking, thereby enhancing construction quality and safety. In this study, a four-factor, four-level orthogonal test was devised, taking into account temperature, confining pressure, dry density, and water content. The complex nonlinear curvilinear relationship between deviatoric stress, volume strain, and axial strain of frozen calcareous clay under different interaction levels was analyzed.