Publications by authors named "D L Logsdon"
Article Synopsis
- Researchers are investigating how trophoblast cells, which are crucial for embryo implantation, grow and differentiate, but understanding is limited.
- Human trophoblast stem cells (TSC) and specific treated stem cells (BAP) are used to study these processes, and their similarities to actual early trophoblasts have been compared.
- The study found that while BAP and TSC closely resemble peri-implantation trophoblasts, they show minor differences in gene expression, suggesting they are useful models for studying implantation without using human embryos.
Background: The fifth-generation SAPIEN 3 Ultra Resilia valve (S3UR) incorporates several design changes as compared with its predecessors, the SAPIEN 3 (S3) and SAPIEN 3 Ultra (S3U) valves, including bovine leaflets treated with a novel process intended to reduce structural valve deterioration via calcification, as well as a taller external skirt on the 29-mm valve size to reduce paravalvular leak (PVL). The clinical performance of S3UR compared with S3 and S3U in a large patient population has not been previously reported.
Objectives: The aim of this study was to compare S3UR to S3/S3U for procedural, in-hospital, and 30-day clinical and echocardiographic outcomes after transcatheter aortic valve replacement (TAVR).
Article Synopsis
- Single-cell analysis of bovine blastocyst transcriptome has identified key cell lineages, including the inner cell mass (ICM), trophectoderm (TE), and transitional cells.
- Comparing embryos derived from in vivo (IVV) and two different in vitro (IVC and IVR) protocols revealed delays in cell fate commitment to ICM in IVC and IVR embryos, affecting their development potential.
- Pathway analysis showed that IVC embryos had reduced cellular signaling and transport activities, leading to lower developmental potential, while IVR embryos exhibited increased signaling but issues with ion balance, resulting in compromised development compared to IVV embryos.
Recent developments in the in vitro culture of peri-implantation stage human embryos have expanded opportunities to investigate implantation stage human development and trophoblast differentiation in the absence of maternal tissues. Emerging single cell omics analyses have offered researchers new tools to explore unanswered biological questions to new depths. In order to investigate the dynamics of human trophoblast cell differentiation during implantation at the single-cell resolution, efficient cell dissociation approaches of trophoblasts from embryos are necessary.
View Article and Find Full Text PDFArticle Synopsis
- Recent advancements in creating human blastoids from naive pluripotent stem cells have improved the efficiency and quantity of these models for studying early human development and implantation.
- The new protocol allowed researchers to conduct proteomics analysis, revealing specific phosphosite signatures important for the signaling processes in human blastoids.
- Additionally, findings indicated that endometrial stromal cells support trophoblast cell survival and growth, while single-cell RNA sequencing helped identify similarities in gene expression between blastoids and blastocysts.