Complement Drives Chronic Inflammation and Progressive Hydrocephalus in Murine Neonatal Germinal Matrix Hemorrhage.

Germinal matrix hemorrhage (GMH) is a pathology that occurs in infancy, with often devastating long-term consequences. Posthemorrhagic hydrocephalus (PHH) can develop acutely, while periventricular leukomalacia (PVL) is a chronic sequala. There are no pharmacological therapies to treat PHH and PVL.

A role for P-selectin and complement in the pathological sequelae of germinal matrix hemorrhage.

Background: Germinal matrix hemorrhage is a devastating disease of pre-term infancy commonly resulting in post-hemorrhagic hydrocephalus, periventricular leukomalacia, and subsequent neurocognitive deficits. We demonstrate vascular expression of the adhesion molecule P-selectin after GMH and investigate a strategy to specifically target complement inhibition to sites of P-selectin expression to mitigate the pathological sequelae of GMH.

Methods: We prepared two fusion proteins consisting of different anti-P-selectin single chain antibodies (scFv's) linked to the complement inhibitor Crry.

A Role for P-selectin and Complement in the Pathological Sequelae of Germinal Matrix Hemorrhage.

Germinal Matrix Hemorrhage is a devastating disease of pre-term infancy commonly resulting in post-hemorrhagic hydrocephalus, periventricular leukomalacia, and subsequent neurocognitive deficits. We demonstrate vascular expression of the adhesion molecule P-selectin after GMH and investigate a strategy to specifically target complement inhibition to sites of P-selectin expression to mitigate the pathological sequelae of GMH. We prepared two fusion proteins consisting of different anti-P-selectin single chain antibodies (scFv's) linked to the complement inhibitor Crry.

Ultra-stable CO-in-water foam by generating switchable Janus nanoparticles in-situ.

Hypothesis: The surface of silica nanoparticles (NP) may be covalently grafted with two amino ligands to balance colloidal stability and interfacial activity via formation of in situ Janus particles. The modified NP may be combined with a like-charged diamine surfactant to create ultra-stable CO foam at low NP concentrations.

Experiments: The NP colloidal stability was measured up to 80 °C in 230 g/L TDS brine with dynamic light scattering.

Effect of interparticle forces on the stability and droplet diameter of Pickering emulsions stabilized by PEG-coated silica nanoparticles.

Hypothesis: Attractive and repulsive interparticle forces influence the stability and structure of Pickering emulsions. The effect these forces have on emulsion behavior must be better understood to improve Pickering emulsions for subsurface applications, including enhanced oil recovery and aquifer decontamination. Past work demonstrates improved emulsion stability with increasing salinity and reduced electrostatic repulsion, possibly because of interparticle networks.