Publications by authors named "D L Ekanayake"
Article Synopsis
- Guinea pigs have been key in biomedical research and typically require sedation for experiments, with the ketamine-xylazine (KX) combination being the standard sedative for years.
- A study explored substituting xylazine with midazolam and examined its combination with ketamine and alfaxalone, finding that KX offered the fastest and longest sedation, while midazolam had shorter duration and increased heart rate effects.
- Despite KX being the most effective, midazolam is not a suitable alternative due to its potential for adverse effects, whereas the midazolam-alfaxalone combination showed a safer profile without significant side effects.
The mechanistic relationships between the progression of growth chondrocyte differentiation, matrix mineralization, oxidative metabolism, and mitochondria content and structure were examined in the ATDC5 murine chondroprogenitor cell line. The progression of chondrocyte differentiation was associated with a statistically significant (p ≤ 0.05) ~2-fold increase in oxidative phosphorylation.
View Article and Find Full Text PDFMycoplasma gallisepticum causes chronic respiratory disease in poultry. A novel vaccine, Vaxsafe MG304 (the ts-304 strain), has greater protective efficacy in chickens than the Vaxsafe MG (strain ts-11) vaccine when delivered by eye drop at 3 weeks of age. Applying this vaccine in the hatchery to 1-day-old birds, using mass administration methods, would improve animal welfare and reduce labour costs associated with handling individual birds.
View Article and Find Full Text PDFGuinea pigs are often used in translational research, but providing them with safe and effective anesthesia is a challenge. Common methods like inhalant anesthesia and injectable ketamine/xylazine induce surgical anesthesia but can negatively affect cardiovascular, respiratory, and thermoregulatory systems and complicate the interpretation of research outcomes. Several alternative anesthetic regimens have been investigated, but none have consistently achieved a surgical plane of anesthesia.
View Article and Find Full Text PDFBackground: There are limited data regarding the safety and immunogenicity of the Sinopharm/BBIBP-CorV vaccine in pregnancy. Therefore, we sought to investigate the antibody responses and maternal and fetal adverse events following this vaccine in pregnant mothers in Sri Lanka.
Methods And Findings: SARS-CoV-2 receptor binding domain (RBD) specific total antibodies and ACE2 blocking antibodies were measured by ELISA in pregnant mothers (n = 94) who received the vaccine in the first (n = 2), second (n = 57) and third (n = 33) trimester of pregnancy.