Diarrheagenic in Stool Specimens Collected from Patients Attending Primary Healthcare Facilities in Ethiopia: Whole-Genome Sequencing-Based Molecular Characterization.

The diarrheagenic (DEC) is the major cause of diarrheal diseases in Africa, including Ethiopia. However, the genetic diversity of pathotypes found in Ethiopia has not been studied well. This study aimed to characterize potential DEC belonging to enteropathogenic (EPEC), Shiga toxin-producing (STEC), enteroaggregative (EAEC), enterotoxigenic (ETEC), and enteroinvasive (EIEC) pathotypes from stool specimens of patients attending primary healthcare units ( = 260) in Addis Ababa and Hossana using whole-genome sequencing.

Genomic Characterization of Extended-Spectrum β-Lactamase-Producing and Third-Generation Cephalosporin-Resistant Isolated from Stools of Primary Healthcare Patients in Ethiopia.

The global spread of antimicrobial resistance genes (ARGs) in is a major public health concern. The aim of this study was to investigate the genomic characteristics of extended-spectrum β-lactamase (ESBL)-producing and third-generation cephalosporin-resistant from a previously obtained collection of 260 isolates from fecal samples of patients attending primary healthcare facilities in Addis Ababa and Hossana, Ethiopia. A total of 29 isolates (19 phenotypically confirmed ESBL-producing and 10 third-generation cephalosporin-resistant isolates) were used.

The Diagnostic Value of qPCR Quantification of in Hive Debris and Adult Bees for Predicting the Onset of American Foulbrood.

American foulbrood (AFB) is a serious infectious disease of honeybees () caused by . Increased count in hive-related material is associated with an increased risk of AFB. Here, we quantified cells in 106 adult bee and 97 hive debris samples using quantitative PCR (qPCR); 66/106 adult bee and 66/97 hive debris samples were collected simultaneously from the same bee colony (paired-sample design).

Case report: First report of potentially zoonotic in a free-living roe deer () in Slovenia.

Adult female and male nematodes were found in the oesophagus of a free-living roe deer () in Slovenia during passive health surveillance of wildlife. The genus was determined by light microscopy based on the genus-specific cuticular bosses in the anterior part of the parasite. Molecular methods were used to confirm the species , which has zoonotic potential.