Detection of herpes simplex virus in first trimester pregnancy loss using molecular techniques.

Background: The etiology of early pregnancy loss is multifactorial and may include viral pathogens. The aim of this study was to investigate the presence of herpes simplex virus (HSV) in gestational tissues of first trimester pregnancy loss using molecular techniques.

Patients And Methods: Gestational tissue from 95 women with first trimester pregnancy loss and 36 women with elective termination of pregnancy was investigated by nested polymerase chain reaction (PCR) and in situ hybridization techniques.

Herpes simplex virus as a determinant risk factor for coronary artery atherosclerosis and myocardial infarction.

Background: Viruses have been detected in atherosclerotic and non-atherosclerotic vascular tissues and may be involved in the mechanisms of atherogenesis. In the present study, we investigated the role of herpes simplex virus (HSV) in the early and late stages of coronary artery atherosclerosis.

Methods And Results: HSV prevalence was investigated in coronary artery samples from 42 autopsy cases, in which death was related to myocardial infarction (MI), and 28 young age autopsy cases without heart disease, who had died from fatal injuries (young victim group), using nested polymerase chain reaction (nPCR) and the highly sensitive in situ hybridization with tyramide signal amplification (ISH-TSA).

Detection of herpes simplex virus, cytomegalovirus, and Epstein-Barr virus in the semen of men attending an infertility clinic.

Objective: To investigate the prevalence of herpes simplex virus (HSV), cytomegalovirus (CMV), and Epstein-Barr virus (EBV) in the semen of men with fertility problems.

Design: A descriptive clinical study.

Settings: Outpatient infertility clinic of a private hospital.

Detection of herpes simplex virus DNA in maternal breast milk by in situ hybridization with tyramide signal amplification.

In this study we investigated the prevalence of herpes simplex virus 1 (HSV-1) and 2 (HSV-2) in maternal breast milk using the technique of in situ hybridization combined with the sensitive detection system of tyramide signal amplification. Breast milk samples were collected from both breasts of 34 puerperals 4-5 days after delivery. HSV DNA was detected in 16 out of 34 examined breast milk specimens.

Detection of herpes simplex virus DNA in human spermatozoa by in situ hybridization technique.

The role of herpes simplex virus (HSV) in male infertility has been investigated using the localizing ability of in situ hybridization technique. Sperm samples obtained from 80 men attending a maternity center because of fertility problems were classified by spermogram and analyzed for the presence of HSV-1 and HSV-2 DNA using digoxigenin-labelled DNA probes. HSV DNA was detected in the nuclei of spermatozoa in 37 semen samples (46%), HSV-1 specifically in 21 cases (26%) and HSV-2 in 16 cases (20%).