Publications by authors named "D Kondoh"
Preassembled complexes of hAgo2 and ssRNA delivered by nanoparticles: a novel silencing gene expression approach overcoming the absence of the canonical pathway of siRNA processing in the apicomplexan parasite Babesia microti, blood parasite of veterinary and zoonotic importance.
Emerg Microbes Infect
December 2025
Article Synopsis
- Current chemical drugs are ineffective for tick control and there are no effective vaccines, leading to the urgent need for new control strategies.
- Small interfering RNAs (siRNAs) have potential to help control parasites, but their effectiveness is limited by challenges in getting them to the RNA-induced silencing complex (RISC).
- This study developed a delivery system using chitosan nanoparticles to successfully transfect siRNAs into parasites, which reduced target gene expression and inhibited parasite growth, opening new avenues for gene therapy and understanding host-parasite interactions.
Article Synopsis
- - The study compares the effects of organ preservation using histidine-tryptophan-ketoglutarate (HTK) and UW solutions, specifically focusing on the ultrastructural changes in hepatocytes after machine perfusion (MP).
- - Ultrastructural evaluations revealed that both hypothermic (HMP) and midthermic (MMP) preservation methods maintained mitochondrial functionality, but HMP showed better results with denser mitochondrial cristae and autophagosome presence after simulated liver graft reperfusion.
- - These results challenge prior conclusions about UW solution, indicating that HMP with HTK may be more effective than MMP, suggesting a need for careful optimization of perfusion conditions before switching preservation methods. *
Most terrestrial mammals have a vomeronasal system to detect specific chemicals. The peripheral organ of this system is a vomeronasal organ (VNO) opening to the incisive duct, and its primary integrative center is an accessory olfactory bulb (AOB). The VNO in seals is thought to be degenerated like whales and manatees, unlike otariids, because of the absence of the AOB.
View Article and Find Full Text PDFFancy breeds of Japanese indigenous chicken display extensive morphological diversity, particularly in tail feathers. Although marked differences in tail and bone traits have been reported between Tosa-jidori (wild type) and Minohikichabo (rich type) breeds, little is known about the pattern of genetic inheritance in cross experiments. Therefore, this study aimed to investigate the strain and sex effects, and inheritance patterns, in the morphometric variation of pygostyle bones among Tosa-jidori, Minohikichabo, and their F hybrids.
View Article and Find Full Text PDFWe developed a novel method for purifying acid-fast bacteria from feces. The method enabled the observation of characteristic clumps of Mycobacterium avium subsp. paratuberculosis (MAP) under electron microscopy by removing contaminants and other bacteria.
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