Publications by authors named "D Klosowska"
Background: Prevalence of type 2 diabetes increases with age. Both the Food and Drug Administration (FDA) and the European Medicines Agency (EMA) promote the enrollment of older patients to randomized controlled trials (RCTs) in diabetes. The objective of this study was to assess the eligibility criteria limiting the inclusion of older adults to RCTs in type 2 diabetes.
View Article and Find Full Text PDFPrevalence of Parkinson's disease (PD) increases with age. The purpose of this study was to evaluate the eligibility criteria in randomized clinical trials (RCTs) in PD, especially those limiting the enrollment of older adults. We examined RCTs of pharmacological and non-pharmacological anti-parkinsonian interventions registered with ClinicalTrials.
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- Breast cancer (BC) is the most prevalent cancer type in women, and this study evaluated eligibility criteria in recent clinical trials that may hinder older patients and those with other health issues from participating.
- The analysis covered 522 systemic anticancer treatment trials conducted between 2020 and 2022, revealing that a significant number (94%) had strict criteria like upper age limits and comorbidity exclusions.
- The findings indicated a notable increase in exclusion criteria for older patients and those with poor performance compared to trials from 2010-2012, suggesting a need for modifications in trial design to be more inclusive.
The objective of this study was to evaluate the effects of A3R phage and lysate obtained after phage infection on neutrophil degranulation. The exocytosis of primary and secondary granules from neutrophils was investigated in vitro in whole blood specimens by flow cytometry based on the expression of specific markers of exocytosis (CD63 for primary granules and CD66b for secondary granules). We found that both A3R and lysate had no significant effect on the exocytosis of primary and secondary granules.
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- A study investigated the effects of T4 bacteriophage and T4-generated E. coli lysate on monocytes, a key type of immune cell involved in fighting bacterial infections.
- Results showed that these preparations increased certain costimulatory molecules (CD40, CD80) and the production of inflammatory cytokines (IL-6 and IL-12) in unactivated monocyte cultures, indicating a heightened immune response.
- However, in cultures activated with LPS (a bacterial component), monocytes did not respond to either T4 or E. coli lysate, suggesting that phage therapy may not overly stimulate the immune system in patients with Gram-negative bacterial infections.