Artificial intelligence based assessment of minimally invasive surgical skills using standardised objective metrics - A narrative review.

Introduction: Many studies display significant heterogeneity in the reliability of artificial intelligence (AI) assessment of minimally invasive surgical (MIS) skills. Our objective is to investigate whether AI systems utilising standardised objective metrics (SOMs) as the basis of skill assessment can provide a clearer understanding of the current state of such technology.

Methods: We systematically searched Medline, Embase, Scopus, CENTRAL and Web of Science from March 2023 to September 2023.

AGS3-based optogenetic GDI induces GPCR-independent Gβγ signaling and macrophage migration.

G protein-coupled receptors (GPCRs) are efficient Guanine nucleotide exchange factors (GEFs) and exchange GDP to GTP on the Gα subunit of G protein heterotrimers in response to various extracellular stimuli, including neurotransmitters and light. GPCRs primarily broadcast signals through activated G proteins, GαGTP, and free Gβγ, and are major disease drivers. Evidence shows that the ambient low threshold signaling required for cells is likely supplemented by signaling regulators such as non-GPCR GEFs and Guanine nucleotide Dissociation Inhibitors (GDIs).

Spatiotemporal Optical Control of Gαq-PLCβ Interactions.

Cells experience time-varying and spatially heterogeneous chemokine signals , activating cell surface proteins including G protein-coupled receptors (GPCRs). The Gαq pathway activation by GPCRs is a major signaling axis with broad physiological and pathological significance. Compared with other Gα members, GαqGTP activates many crucial effectors, including PLCβ (Phospholipase Cβ) and Rho GEFs (Rho guanine nucleotide exchange factors).

Spatiotemporal optical control of Gαq-PLCβ interactions.

Cells experience time-varying and spatially heterogeneous chemokine signals in vivo, activating cell surface proteins, including G protein-coupled receptors (GPCRs). The Gαq pathway activation by GPCRs is a major signaling axis with a broad physiological and pathological significance. Compared to other Gα members, GαqGTP activates many crucial effectors, including PLCβ (Phospholipase Cβ) and Rho GEFs (Rho guanine nucleotide exchange factors).