How do Gram-negative bacteria escape predation by Bdellovibrio bacteriovorus?

Bdellovibrio bacteriovorus is a small predatory bacterium which reproduces by invading and killing Gram-negative bacteria. The natural antimicrobial activity of B. bacteriovorus has garnered interest for the potential to develop this predatory bacterium as a therapeutic agent.

recovered from the lung of a harbour porpoise, and comparative analyses with spp.

Strain M1325/93/1 (herein referred to by our laboratory identifier, GFKo1) of was isolated from the lung of a harbour porpoise in 1993. The genome sequence and antimicrobial resistance profile (genomic, phenotypic) of the strain were generated, with the genomic data compared with those from closely related bacteria. We demonstrate that the recently described chromosomally encoded AmpC β-lactamase is a core gene of , and suggest that new variants of this class of lactamase are encoded by other members of the genus .

Complete genome sequence of strain DSM 15096.

We present the first complete genome sequence of the species . Strain DSM 15096 was sequenced with a hybrid approach using Oxford Nanopore Technologies long-read sequencing and Illumina short-read sequencing. The assembled sequences produced a 2 808 898 bp chromosomal molecule containing 2705 predicted genes, plus eight plasmids.

Lytic bacteriophage vB_KmiS-Kmi2C disrupts biofilms formed by members of the Klebsiella oxytoca complex, and represents a novel virus family and genus.

Aims: This study aimed to characterize the lytic phage vB_KmiS-Kmi2C, isolated from sewage water on a GES-positive strain of Klebsiella michiganensis.

Methods And Results: Comparative phylogenetic and network-based analyses were used to characterize the genome of phage vB_KmiS-Kmi2C (circular genome of 42 234 bp predicted to encode 55 genes), demonstrating it shared little similarity with other known phages. The phage was lytic on clinical strains of K.

Extended genomic analyses of the broad-host-range phages vB_KmiM-2Di and vB_KmiM-4Dii reveal slopekviruses have highly conserved genomes.

High levels of antimicrobial resistance among members of the complex (KoC) have led to renewed interest in the use of bacteriophage (phage) therapy to tackle infections caused by these bacteria. In this study we characterized two lytic phages, vB_KmiM-2Di and vB_KmiM-4Dii, that were isolated from sewage water against two GES-5-positive strains (PS_Koxy2 and PS_Koxy4, respectively). ViPTree analysis showed both phages belonged to the genus gene-based sequence analysis of 108 presumptive isolates (=59 clinical, =49 veterinary) found to be more prevalent (46 % clinical and 43 % veterinary, respectively) than (40 % clinical and 6 % veterinary, respectively).