Endothelial-specific knockout of the scramblase TMEM16F impairs in vivo clot formation.

Objective: Loss of function of the phospholipid scramblase (PLS) TMEM16F results in Scott Syndrome, a hereditary bleeding disorder generally attributed to intrinsic platelet dysfunction. The role of TMEM16F in endothelial cells, however, is not well understood. We sought to test the hypothesis that endothelial TMEM16F contributes to hemostasis by measuring bleeding time and venous clotting in endothelial-specific knockout (ECKO) mice.

Nano-biopesticide formulation comprising of silver nanoparticles anchored to Ocimum sanctum: a sustainable approach to pest control in jute farming.

The jute hairy caterpillar, Spilosoma obliqua (Lepidoptera: Erebidae) is considered as one of the major threats to jute cultivation. The best eco-friendly methods to combat these jute pests involve administration of nano-biopesticides, as a successful alternative to the toxic chemicals. In this study, a nano-biopesticide formulation containing green synthesized silver nanoparticles (Ag NPs) using Ocimum sanctum leaf extract has been proposed.

SLAM/SAP signaling regulates discrete γδ T cell developmental checkpoints and shapes the innate-like γδ TCR repertoire.

During thymic development, most γδ T cells acquire innate-like characteristics that are critical for their function in tumor surveillance, infectious disease, and tissue repair. The mechanisms, however, that regulate γδ T cell developmental programming remain unclear. Recently, we demonstrated that the SLAM/SAP signaling pathway regulates the development and function of multiple innate-like γδ T cell subsets.

Early adipose tissue wasting in a novel preclinical model of human lung cancer cachexia.

Cancer cachexia (CC), a syndrome of skeletal muscle and adipose tissue wasting, reduces responsiveness to therapies and increases mortality. There are no approved treatments for CC, which may relate to discordance between pre-clinical models and human CC. To address the need for clinically relevant models, we generated tamoxifen-inducible, epithelial cell specific ( ) mice.

Distinct Inflammatory Programs Underlie the Intramuscular Lipid Nanoparticle Response.

Developments in mRNA/lipid nanoparticle (LNP) technology have advanced the fields of vaccinology and gene therapy, raising questions about immunogenicity. While some mRNA/LNPs generate an adjuvant-like environment in muscle tissue, other mRNA/LNPs are distinct in their capacity for multiple rounds of therapeutic delivery. We evaluate the adjuvancy of components of mRNA/LNPs by phenotyping cellular infiltrate at injection sites, tracking uptake by immune cells, and assessing the inflammatory state.