Publications by authors named "D Jansova"
Article Synopsis
- Mammalian oocyte development relies on precise translation of maternal mRNA during critical phases of meiotic and early embryonic stages when transcription stops.
- The protein cytoplasmic polyadenylation element-binding protein 3 (CPEB3) plays a crucial role by stabilizing specific mRNAs needed for embryonic transcription.
- Without CPEB3, oocytes can undergo meiosis but face early embryonic development issues due to disrupted mRNA stability, leading to protein expression problems and failed embryonic transcription initiation.
Nucleic Acids Res
December 2023
Article Synopsis
- Translation is essential for development, particularly during the silenced transcription phase of oocytes and early embryos.
- A genome-wide translatome analysis revealed that while global protein synthesis decreases during M-phases, the initiation and elongation of translation are activated, showing a dynamic shift in how specific mRNAs are translated.
- This study provides new insights into gene expression regulation during oocyte meiosis and the first two embryonic mitoses, marking a significant step towards understanding the molecular mechanisms of translation control in early development.
J Mol Biol
September 2021
Article Synopsis
- - During oocyte growth, cells accumulate RNAs essential for oocyte and embryo development, even as transcription stops.
- - A new technique was developed to analyze the localization and translation of specific mRNAs in mouse oocytes and embryos, revealing localized translation patterns with important regulatory roles.
- - The study found that certain mRNAs, like CyclinB1 and Mos, are concentrated in the cytoplasm of fully grown oocytes and are translated significantly at specific cellular locations, providing insights into molecular processes during cell development.
Article Synopsis
- * The study identifies that p38 mitogen-activated protein kinases (p38-MAPKs) are active early in blastocyst development, regulating essential processes like ribosome function and protein translation necessary for PrE differentiation.
- * Inhibiting p38-MAPK affects PrE development but can be partly reversed by activating mTOR, whereas similar effects from inhibiting the ERK pathway linked to FGF4 are not reversed by mTOR, highlighting p38-MAPK's unique role in PrE development. *
Article Synopsis
- Increasing maternal age in mammals leads to lower quality oocytes, higher aneuploidy rates, and reduced developmental ability.
- Research shows that many mRNAs are translated differently in oocytes from older females compared to younger ones, particularly affecting those related to the cell cycle.
- Specific proteins like CASTOR1 and SGK1 are linked to errors in chromosome alignment during meiosis, indicating that improper translation of proteins at the start of meiosis is a factor in the age-related decline in reproductive success.
