Publications by authors named "D J Meisinger"
Article Synopsis
- Glycosylation is crucial for modifying lipids and sorting proteins, with its regulation involving a unique distribution of enzymes in the Golgi and the action of SPPL3.
- In cells lacking the retention factor LYSET/TMEM251, there is increased secretion of a Golgi protein, B4GALT5, due to disrupted M6P tagging, which typically marks proteins for lysosomal degradation.
- The study reveals that GOLPH3 and GOLPH3L adaptors play a critical role in stabilizing the LYSET-GNPT complex, maintaining proper Golgi function, and ensuring efficient lysosomal enzyme processing.
Article Synopsis
- Golgi membrane proteins like glycosyltransferases play a crucial role in modifying proteins and lipids through glycosylation.
- SPPL3, an intramembrane protease, specifically cleaves select Golgi enzymes, influencing their secretion and the overall glycosylation process in cells, though the full range of its substrates is not completely known.
- This study identifies over 20 novel SPPL3 substrates using specialized cell lines and techniques, emphasizing the importance of SPPL3 in enzyme turnover and regulation of glycosylation pathways.
Microbial mats in sulfidic cave streams offer unique opportunities to study redox-based biogeochemical nutrient cycles. Previous work from Lower Kane Cave, Wyoming, USA, focused on the aerobic portion of microbial mats, dominated by putative chemolithoautotrophic, sulfur-oxidizing groups within the Epsilonproteobacteria and Gammaproteobacteria. To evaluate nutrient cycling and turnover within the whole mat system, a multidisciplinary strategy was used to characterize the anaerobic portion of the mats, including application of the full-cycle rRNA approach, the most probable number method, and geochemical and isotopic analyses.
View Article and Find Full Text PDFLower Kane Cave, Wyoming (USA), has hydrogen sulfide-bearing springs that discharge into the cave passage. The springs and cave stream harbour white filamentous microbial mats dominated by Epsilonproteobacteria. Recently, novel 16S rRNA gene sequences from the phylum Acidobacteria, subgroup 7, were found in these cave mats.
View Article and Find Full Text PDFTo evaluate factors affecting pork color, the gluteus medius (GM), longissimus lumborumetthoracis (LT), semimembranosus (SM), biceps femoris (BF), and triceps brachii (TRI) muscles from pork carcasses of varying ultimate pHs were allowed to bloom for 30 min. L*, a*, and b* values, hue angle, chroma and visual color were determined. Color was evaluated using HunterLab (illuminants A, C, D(65) and F) and Minolta (illuminants C and D(65)) Spectrocolorimeters.
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