Publications by authors named "D J Kyle"
Article Synopsis
- - Malaria, caused by the Plasmodium parasite, faces challenges due to drug resistance, prompting the need for new treatments targeting both symptomatic and asymptomatic liver stages; a cost-effective luciferase detection method for liver stage screening has been developed using common lab reagents.
- - The study optimized a protocol for testing liver stage parasites, validating it with 28 existing anti-malarials to ensure reliable signal data, enabling the screening of the Global Health Priority Box (GHPB) for potential drug candidates.
- - Results showed that the optimized screening method produced a more stable luciferase signal with lower cell density, identifying 9 promising hits with selective activity against the P. berghei liver stages.
parasites undergo development and replication within the hepatocytes before infecting the erythrocytes and initiating clinical malaria. Although type-I interferons (IFNs) are known to hinder infection within the liver, the underlying mechanisms remain unclear. Here, we describe two IFN-I-driven hepatocyte antimicrobial programs controlling liver-stage malaria.
View Article and Find Full Text PDFBackground And Objectives: Dystonia is a common, debilitating, and often treatment refractory motor symptom of cerebral palsy (CP), affecting 70-80% of this population based on research assessments. However, routine clinical evaluation for dystonia in CP has failed to match these expected numbers. Addressing this diagnostic gap is a medical imperative because the presence of dystonia rules in or out certain treatments for motor symptoms in CP.
View Article and Find Full Text PDFBackground: The loss of ancestral microbes, or the "disappearing microbiota hypothesis" has been proposed to play a critical role in the rise of inflammatory and immune diseases in developed nations. The effect of this loss is most consequential during early-life, as initial colonizers of the newborn gut contribute significantly to the development of the immune system.
Methods: In this longitudinal study (day 3, week 3, and month 3 post-birth) of infants of Asian ancestry born in Singapore, we studied how generational immigration status and common perinatal factors affect bifidobacteria and subsp.
The increase in research funding for the development of antimalarials since 2000 has led to a surge of new chemotypes with potent antimalarial activity. High-throughput screens have delivered several thousand new active compounds in several hundred series, including the 4,7-diphenyl-1,4,5,6,7,8-hexahydroquinolines, hereafter termed dihydropyridines (DHPs). We optimized the DHPs for antimalarial activity.
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