Downregulation of the immunoproteasome subunit PSMB8 attenuates sepsis-associated acute kidney injury through the NF-κB pathway.

Sepsis-associated acute kidney injury (S-AKI) is a prevalent and life-threatening complication in hospitalized and critically ill patients. Recent researches indicates that immunoproteasome, especially proteasome 20S subunit beta 8 (PSMB8), is highly associated with various kidney diseases. This study aims to investigate the potential involvement of PSMB8 in S-AKI and its impact on apoptosis and inflammation.

Quantitative profiling of human translation initiation reveals elements that potently regulate endogenous and therapeutically modified mRNAs.

mRNA therapeutics offer a potentially universal strategy for the efficient development and delivery of therapeutic proteins. Current mRNA vaccines include chemically modified nucleotides to reduce cellular immunogenicity. Here, we develop an efficient, high-throughput method to measure human translation initiation on therapeutically modified as well as endogenous RNAs.

[Enzymatic reaction pattern of saponins in Gynostemma pentaphyllum and identification of their transformed products].

The activity of rare saponins is generally better than that of their prototypes. There are significant differences in the proportions and pharmacological effects of rare saponins/prototype saponins in Gynostemma pentaphyllum samples dried with different methods, which may be related to the reaction catalyzed by glucosidase. To explore the pattern of the enzymatic reaction catalyzed by glucosidase during the processing of G.

A nitrogen-metabolism inhibitor NmrA regulates conidial production, melanin synthesis and virulence in phytopathogenic fungus .

NmrA homologs have been reported as conserved regulators of the nitrogen metabolite repression (NMR) in various fungi. Here, we identified a NmrA homolog in and reported its functions in nitrogen utilization, growth and development, and pathogenesis. VdNmrA interacts with AreA protein and regulates the expression of a typical NCR target, the formamidase gene.