Nucleic acid separations using superficially porous silica particles.

Ion pair reverse-phase liquid chromatography has been widely employed for nucleic acid separations. A wide range of alternative stationary phases have been utilised in conjunction with ion pair reverse-phase chromatography, including totally porous particles, non-porous particles, macroporous particles and monolithic stationary phases. In this study we have utilised superficially porous silica particles in conjunction with ion pair reverse-phase liquid chromatography for the analysis of nucleic acids.

Vasculitic rash: do not jump to conclusions.

A 57-year-old man presented with a vasculitic leg rash, weight loss and evidence of blood and protein in the urine. He was initially thought to have Henoch-Schönlein purpura but was eventually diagnosed with bacterial endocarditis.

Resolution of positional isomers by capillary electrochromatography.

Electroseparations have been very successful in increasing efficiencies and reducing analysis times. The analytical technique originally applied to open tube capillaries (capillary electrophoresis) has been used as a basis to develop renewed interest in electrochromatography. This paper describes the use of capillary electrochromatography to separate two positional isomers and describes a comparison between gas chromatography (GC), capillary electrochromatography (CEC) and nano-HPLC.