Publications by authors named "D Helm"
Ductular reaction (DR) is the hallmark of cholestatic diseases manifested in the proliferation of bile ductules lined by biliary epithelial cells (BECs). It is commonly associated with an increased risk of fibrosis and liver failure. The receptor for advanced glycation end products (RAGE) was identified as a critical mediator of DR during chronic injury.
View Article and Find Full Text PDFQuality control procedures play a pivotal role in ensuring the reliability and consistency of data generated in mass spectrometry-based proteomics laboratories. However, the lack of standardized quality control practices across laboratories poses challenges for data comparability and reproducibility. In response, we conducted a harmonization study within proteomics laboratories of the Core for Life alliance with the aim of establishing a common quality control framework, which facilitates comprehensive quality assessment and identification of potential sources of performance drift.
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- BK polyomavirus-associated nephropathy (BKPyVAN) is a significant complication after kidney transplantation, usually managed by reducing immunosuppression when BK polyomavirus (BKPyV) is detected.
- A study involving 1,076 kidney transplant recipients found a higher risk of developing de novo donor-specific antibodies (dnDSA) in patients with high BKPyV DNA loads compared to those without, suggesting that the required immunosuppression reduction can worsen immune responses.
- While high BKPyV DNAemia increased dnDSA risk, there was no significant difference in the occurrence of biopsy-proven acute rejection (BPAR) between groups, indicating a need for better strategies to manage BKPyV infections in transplant patients.
Glioblastoma is a highly aggressive brain tumor for which there is no cure. The dire prognosis of this disease is largely attributable to a high level of heterogeneity, including the presence of a subpopulation of tumor-initiating glioblastoma stem-like cells (GSCs), which are refractory to chemo- and radiotherapy. Here, in an unbiased marine-derived fungal extract screen, together with bioguided dereplication based on high-resolution mass spectrometry, we identified malformin C to preferentially induce cell death in patient-derived GSCs and explore the potential of this cyclic peptide as a therapeutic agent for glioblastoma.
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- - This study examines lesioned fascicles in the sciatic nerves of people with diabetic neuropathy (DN) to understand how these lesions relate to clinical symptoms and their underlying mechanisms.
- - Using advanced imaging and proteomic analysis, researchers found that only individuals with type 2 diabetes (T2D) had these lesions, which showed significant damage like axonal degeneration and demyelination, along with a compromised blood nerve barrier (BNB).
- - The results indicate that while non-lesioned fascicles from T2D donors showed neuroprotective responses, lesioned ones did not and had increased inflammatory activity, suggesting a harmful connection between the liver and nerves that could be targeted for therapy.