Publications by authors named "D H Leach"

Current plasmid propagation in E. coli compromises large inverted repeats, such as inverted terminal repeats (ITRs) of adeno-associated virus (AAV). Direct long-read sequencing analyses upon varying strains and culture conditions revealed ITR instability caused by a slipped misalignment mechanism, although other mechanism probably contribute.

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( ) is the causative agent of tuberculosis (TB), the leading cause of infectious-disease related deaths worldwide. TB infections present as a spectrum from active to latent disease. In the human host, faces hostile environments, such as nutrient deprivation, hypoxia, and low pH.

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Article Synopsis
  • Branched DNA molecules play a critical role in DNA processes like replication and repair, making their structural understanding essential for grasping these biological mechanisms.
  • While gel electrophoresis offers a bulk analysis of DNA, direct visualization at the single-molecule level is necessary but requires pure samples free from unwanted DNA interference.
  • The study focuses on overcoming challenges in isolating large, branched DNA structures, specifically in E. coli, by utilizing techniques like β-agarase digestion and multiple rounds of gel electrophoresis for enhanced sample purity.
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In most patients with advanced prostate cancer treated with hormonal therapy, androgen independence eventually emerges, leading to death. Androgen receptor signalling remains an important prostate cancer driver, even in the advanced disease stage. MicroRNAs (miRs), non-coding RNAs that regulate gene expression by inhibiting translation and/or promoting degradation of target mRNAs, can act as tumour suppressors or "oncomiRs" and modulate tumour growth.

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