Dysregulation of PI4P in the trans Golgi regions activates the mammalian Golgi stress response.

The Golgi stress response is an important cytoprotective system that enhances Golgi function in response to cellular demand, while cells damaged by prolonged Golgi stress undergo cell death. OSW-1, a natural compound with anticancer activity, potently inhibits OSBP that transports cholesterol and phosphatidylinositol-4-phosphate (PI4P) at contact sites between the endoplasmic reticulum and the Golgi apparatus. Previously, we reported that OSW-1 induces the Golgi stress response, resulting in Golgi stress-induced transcription and cell death.

Does the flutter kick increase hand propulsion in front crawl swimming?

This study aimed to investigate the effect of the flutter kick on the propulsive force generated by a stroke. Eight male swimmers performed 20 m front crawl trials under two conditions: the Whole Condition, involving maximum effort (T100%) and stroke frequencies at 70, 80, and 90% of T100%, and the Arm Condition, which excluded the flutter kick and matched stroke frequencies with the Whole Condition. Various parameters, including swimming velocity, stroke frequency, stroke length, three-dimensional (3D) resultant hand speed, and hand propulsion were calculated based on underwater 3D motion analysis and hand pressure distribution measurements.

Detecting single-point isomeric differences in polymer chains by MOF column chromatography.

Liquid chromatography with a metal-organic framework (MOF) as the stationary phase enables nanopore threading-based recognition of polymers and identification of single-point isomeric structural differences in the polymer main chain. The polymer adsorption affinity to the MOF and transient kinetics of polymer insertion into the nanopores play crucial roles in the recognition process.

Identification and characterization of an enhancer element regulating expression of Cdkn1c (p57 gene).

The mammalian p57 protein is a member of the CIP/KIP family of cyclin-dependent kinase inhibitors and plays an essential role in the development of multiple tissues during embryogenesis as well as in the maintenance of tissue stem cells in adults. Although several transcription factors have been implicated in regulating the p57 gene, cis-elements such as enhancers that regulate its expression have remained ill-defined. Here we identify a candidate enhancer for the mouse p57 gene (Cdkn1c) within an intron of the Kcnq1 locus by 4C-seq analysis in mouse embryonic stem cells (mESCs).

Secretion of endoplasmic reticulum protein VAPB/ALS8 requires topological inversion.

VAMP-associated protein (VAP) is a type IV integral transmembrane protein at the endoplasmic reticulum (ER). Mutations in human VAPB/ALS8 are associated with amyotrophic lateral sclerosis (ALS). The N-terminal major sperm protein (MSP) domain of VAPB (Drosophila Vap33) is cleaved, secreted, and acts as a signaling ligand for several cell-surface receptors.