Quantification of short echo time MRS signals with improved version of QUantitation based on quantum ESTimation algorithm.

Magnetic resonance spectroscopy offers information about metabolite changes in the organism, which can be used in diagnosis. While short echo time proton spectra exhibit more distinguishable metabolites compared with proton spectra acquired with long echo times, their quantification (and providing estimates of metabolite concentrations) is more challenging. They are hampered by a background signal, which originates mainly from macromolecules (MM) and mobile lipids.

Cholinergic Neurotransmission in the Posterior Insular Cortex Is Altered in Preclinical Models of Neuropathic Pain: Key Role of Muscarinic M2 Receptors in Donepezil-Induced Antinociception.

Unlabelled: Neuropathic pain is one of the most debilitating pain conditions, yet no therapeutic strategy has been really effective for its treatment. Hence, a better understanding of its pathophysiological mechanisms is necessary to identify new pharmacological targets. Here, we report important metabolic variations in brain areas involved in pain processing in a rat model of oxaliplatin-induced neuropathy using HRMAS (1)H-NMR spectroscopy.

Quantification in magnetic resonance spectroscopy based on semi-parametric approaches.

Magnetic resonance spectroscopy (MRS) is a value-added modality to magnetic resonance imaging (MRI) that is often used in diagnosis, treatment and progression monitoring, as well as in non-destructive, non-invasive studies of disease states in humans and model systems in animals. The availability of high magnetic field strengths and use of hyperpolarized nuclei, combined with the possibility of acquiring spectra at very short echo-time, have dramatically increased the potential of MRS. For the last two decades, a challenge has been to quantify short echo-time proton spectra that exhibit many metabolites, and to estimate their concentrations.

Optimized quantitative magnetic resonance spectroscopy for clinical routine.

Several practical obstacles in data handling and evaluation complicate the use of quantitative localized magnetic resonance spectroscopy (qMRS) in clinical routine MR examinations. To overcome these obstacles, a clinically feasible MR pulse sequence protocol based on standard available MR pulse sequences for qMRS has been implemented along with newly added functionalities to the free software package jMRUI-v5.0 to make qMRS attractive for clinical routine.

In vivo proton HR-MAS NMR metabolic profile of the freshwater cladoceran Daphnia magna.

The method concerning in vivo proton HR-MAS NMR metabolic profiling of the freshwater cladoceran Daphnia magna is presented. Viability tests of D. magna under different spinning rates were performed.