N-glycan core tri-fucosylation requires Golgi α-mannosidase III activity that impacts nematode growth and behavior.

N-glycans with complex core chitobiose modifications are observed in various free-living and parasitic nematodes but are absent in mammals. Using Caenorhabditis elegans as a model, we demonstrated that the core N-acetylglucosamine (GlcNAc) residues are modified by three fucosyltransferases (FUTs), namely FUT-1, FUT-6, and FUT-8. Interestingly, FUT-6 can only fucosylate N-glycans lacking the α1,6-mannose upper arm, indicating that a specific α-mannosidase is required to generate substrates for subsequent FUT-6 activity.

Dual-Acting Nitric Oxide Donor and Phosphodiesterase Inhibitor TOP-N53 Increases Lifespan and Health Span of .

The quest for extending lifespan and promoting a healthy aging has been a longstanding pursuit in the field of aging research. The control of aging and age-related diseases by nitric oxide (NO) and cGMP signaling is a broadly conserved process from worms to human. Here we show that TOP-N53, a dual-acting NO donor and PDE5 inhibitor, can increase both lifespan and health span in .

Multisite regulation integrates multimodal context in sensory circuits to control persistent behavioral states in C. elegans.

Maintaining or shifting between behavioral states according to context is essential for animals to implement fitness-promoting strategies. How the integration of internal state, past experience and sensory inputs orchestrates persistent multidimensional behavioral changes remains poorly understood. Here, we show that C.

Multiple antagonist calcium-dependent mechanisms control CaM kinase-1 subcellular localization in a thermal nociceptor.

Nociceptive habituation is a conserved process through which pain sensitivity threshold is adjusted based on past sensory experience and which may be dysregulated in human chronic pain conditions. Noxious heat habituation in involves the nuclear translocation of CaM kinase-1 (CMK-1) in the FLP thermo-nociceptors neurons, causing reduced animal heat sensitivity and avoidance responses. The phosphorylation of CMK-1 on T179 by CaM kinase kinase-1 (CKK-1) is required for nuclear entry.

Gendered ethnic choice effects at the transition to upper secondary education in Switzerland.

Positive ethnic choice effects, namely a higher likelihood of attending more demanding educational tracks among students of immigrant origin compared to their native peers, are observed in many countries. Immigrant optimism, and thus the striving for upward social mobility, is seen as a key mechanism for explaining ethnic choice effects. However, research on this topic often ignores gendered educational pathways and trajectories.