Publications by authors named "D Gautheret"
Antecedent viral immunization and efficacy of immune checkpoint blockade: an extensive serum antibody profile to predict outcomes in non-small cell lung cancer.
J Immunother Cancer
November 2024
Introduction: Immune checkpoint blockers (ICBs) revolutionized the treatment of patients with advanced non-small cell lung cancer (NSCLC) but only a fraction of them obtain a response, and clinical benefit from these treatments is often difficult to predict. The aim of our study is to unveil the potential implications of antibody response to previous viral infections in predicting response to ICBs in patients with NSCLC.
Methods: Sera from patients treated with ICBs alone, chemotherapy (CT) or a combination of CT-ICBs were analyzed with VirScan (CDI Labs, USA), a high-throughput method that comprehensively analyzes epitope-level antiviral IgG antibodies via programmable phage display and immunoprecipitation sequencing.
is the leading cause of healthcare-associated diarrhea in industrialized countries. Many questions remain to be answered about the mechanisms governing its interaction with the host during infection. Non-coding RNAs (ncRNAs) contribute to shape virulence in many pathogens and modulate host responses; however, their role in infection (CDI) has not been explored.
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- The study investigated the significance of metabolic tumor volume (tMTV) in patients with advanced non-small cell lung cancer (NSCLC) undergoing immune checkpoint blockade (ICB) therapy, using 18F-FDG-PET/CT scans.
- It involved 518 patients across multiple institutions and found that those with high tMTV had poorer overall survival when treated with ICBs alone compared to those with low tMTV.
- The research suggests that high tMTV is associated with increased systemic inflammation and genomic instability, making it a potential biomarker for determining treatment strategies in NSCLC patients with positive PD-L1 expression.
Article Synopsis
- K-mer indexing is an effective technique for searching RNA sequences in RNA-seq libraries but hasn't allowed for direct quantification of RNA until now.
- The study demonstrates that arbitrary RNA sequences can be quantified quickly and accurately by breaking them down into k-mers, achieving precision similar to traditional methods.
- Using an extensive RNA-seq sample collection from the Cancer Cell Line Encyclopedia, the researchers showcase how k-mer indexing can uncover non-reference sequences and variant RNAs related to specific genetic changes.
Article Synopsis
- Understanding RNA targeting to membraneless organelles like P-bodies (PBs) is crucial for revealing their functions in cells.
- This study shows that PBs in HEK293 cells undergo significant changes in RNA content throughout the cell cycle, with different mRNA localization patterns appearing at various stages (G1, S, G2).
- The findings suggest that PBs actively sort mRNAs based on their translation status and characteristics, implying that they play a more dynamic role than merely housing excess untranslated mRNAs.