Publications by authors named "D G Littlewood"

Article Synopsis
  • Soil-transmitted helminths (STHs) infect about 18% of the global population, presenting a health challenge and highlighting the limitations of traditional microscopy for diagnostics, especially in low-prevalence areas.
  • This study compared the effectiveness of different quantitative real-time PCR (qPCR) methods for detecting STH DNA in stool samples spiked with known quantities of STH eggs and larvae.
  • Results showed a strong correlation between qPCR outcomes and egg/larvae counts for most STHs tested, confirming qPCR as a reliable diagnostic tool, yet also indicating variability between methods and suggesting the need for further research to improve accuracy in parasite burden quantification.
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Article Synopsis
  • White-tailed antsangies, a type of Madagascan rodent, were found to have an outbreak of H. nana, a type of tapeworm, marking the first recorded case in this species.
  • The examination of the affected rodents revealed cysticerci (larval form of the tapeworm) in multiple organs, including the intestines and liver, and identified gross lesions through histopathological methods.
  • DNA testing confirmed the presence of H. nana in all cases, suggesting that these infections may lead to unique pathological features like visceral larva migrans in white-tailed antsangies, different from typical rodent hosts.
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Partial mitochondrial cox1 gene sequences from four recently recognised European species of terrestrial planarians, and ribosomal ITS1 sequences for two of them, are presented: Marionfyfea adventor, Artioposthia exulans (both introduced from New Zealand), Australopacifica atrata (from Australia) and specimens putatively identified as Microplana edwardsi, presumed to be native to the UK. The sequences are compared with those from other terrestrial planarian species and analysed phylogenetically. Results indicate that the sister group of M.

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The identification of gastrointestinal helminth infections of humans and livestock almost exclusively relies on the detection of eggs or larvae in faeces, followed by manual counting and morphological characterisation to differentiate species using microscopy-based techniques. However, molecular approaches based on the detection and quantification of parasite DNA are becoming more prevalent, increasing the sensitivity, specificity and throughput of diagnostic assays. High-throughput sequencing, from single PCR targets through to the analysis of whole genomes, offers significant promise towards providing information-rich data that may add value beyond traditional and conventional molecular approaches; however, thus far, its utility has not been fully explored to detect helminths in faecal samples.

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