Chromatography analysis of seminal plasma proteins in buffalo semen samples with high and low cryotolerance.

The aim of this study was to analyse and identify specific buffalo seminal plasma proteins (SPPs) responsible for sperm cryotolerance during low temperature storage. Computer Assisted Sperm Analysis (CASA) of the motility and viability of buffalo spermatozoa was performed before freezing and after thawing. Two sample groups were formed - ejaculates with high cryotol- erance (group A) and low cryotolerance (group B).