Endothelial cell proliferation during angiogenesis. In vitro modulation by basement membrane components.

Modulation of the behavior of microvascular endothelial cells during angiogenesis has been observed to correlate with changes in the extracellular matrix. These reports prompted a comparison of the growth of microvascular endothelial cells on monolayers of various matrix components in vitro. Over a 5 day period, the proliferation of these cells was significantly greater on laminin than on either plasma fibronectin, the interstitial collagen types I and III, or on the basement membrane collagen type IV.

Pathogenesis of desmoplasia. I. Immunofluorescence identification and localization of some structural proteins of line 1 and line 10 guinea pig tumors and of healing wounds.

The structural proteins of the scirrhous line 1 and the medullary line 10 bile duct carcinomas, both syngeneic in strain 2 Sewall-Wright inbred guinea pigs, were studied. Tumor structural proteins were compared with those of healing wounds. A provisional stromal matrix of cross-linked fibrin and fibronectin was initially deposited in both tumors and wounds and was subsequently replaced by granulation tissue containing collagen types I and III.

Pathogenesis of tumor desmoplasia. II. Collagens synthesized by line 1 and line 10 guinea pig carcinoma cells and by syngeneic fibroblasts in vitro.

For the investigation of the pathogenesis of desmoplasia, the capacities to synthesize collagen in vitro of 2 bile duct carcinomas (lines 1 and 10) of Sewall-Wright inbred strain 2 guinea pigs and of syngeneic dermal fibroblasts were studied. Line 10 cells synthesized collagen type IV as judged by sensitivity to bacterial collagenase, by immunoprecipitation, by migration of pro alpha (IV) chains and pepsin-resistant fragments on sodium dodecyl sulfate-polyacrylamide gels, and by immunofluorescence. Line 1 cells also synthesized small amounts of collagenase-sensitive protein.