Single-cycle parainfluenza virus type 5 vectors for producing recombinant proteins, including a humanized anti-V5 tag antibody.

Parainfluenza virus type 5 (PIV5) can cause either persistent or acute/lytic infections in a wide range of mammalian tissue culture cells. Here, we have generated PIV5 fusion (F)-expressing helper cell lines that support the replication of F-deleted viruses. As proof of the principle that F-deleted single-cycle infectious viruses can be used as safe and efficient expression vectors, we have cloned and expressed a humanized (Hu) version of the mouse anti-V5 tag antibody (clone SV5-Pk1).

Baseline Characteristics of Self-Determination Theory Constructs and Accelerometer-Derived Physical Activity: The Exercise Promotion in Primary Care (EPPC) Trial.

Background: Health behavior change theories provide a conceptual basis to promote physical activity, one of which is the Self-Determination Theory (SDT). This cross-sectional study compared SDT constructs, specifically exercise goal setting, exercise planning, and outcome expectations, with objectively assessed Moderate-To-Vigorous Physical Activity (MVPA) among a demographically diverse cohort of adults.

Methods: Participants were 18 to 74 years with prediabetes or type 2 diabetes not prescribed insulin and were physically inactive by self-report.

Pediatric cardiac surgical site infections: A single-center quality improvement initiative.

Objective: Pediatric cardiac surgery site infections (SSI) represent significant morbidity. Our institution reported elevated SSI rates of 3.48 per 100 cases over a 5-year period above target rates of 2.

Lessons Learned from a Quality Improvement Initiative to Increase COVID-19 Vaccination in Hospitalized Children.

Introduction: Pediatric coronavirus disease 2019 (COVID-19) vaccination rates in the United States remain lower compared with adults. We aimed to (1) implement a quality improvement initiative to increase COVID-19 vaccination 2-fold in hospitalized patients 12-21 years of age from 4.7% during the baseline period (August 10, 2021-November 1, 2021) to 9.

Elucidation of Antimicrobials and Biofilm Inhibitors Derived from a Polyacetylene Core.

The development of new antibiotics with unique mechanisms of action is paramount to combating the growing threat of antibiotic resistance. Recently, based on inspiration from natural products, an asymmetrical polyacetylene core structure was examined for its bioactivity and found to have differential specificity for different bacterial species based on the substituents around the conjugated alkyne. This research further probes the structural requirements for bioactivity through a systematic synthesis and investigation of new compounds with variable carbon chain length, alkynyl subunits, and alcohol substitution.