Establishment and evaluation of a method for measuring ornithine transcarbamylase activity in micro blood of neonates.

Background: Ornithine transcarbamylase deficiency exhibits a high degree of clinical heterogeneity, making its screening and classification challenging in some instances. In this study, we first established a simple and stable method for testing ornithine transcarbamylase activity using micro blood from newborns, rather than relying on venous blood.

Methods: The activity of ornithine transcarbamylase was assessed by measuring the concentration of citrulline produced in the reaction with carbamoyl phosphate and ornithine, using serum, plasma or micro blood.

Association between auditory mismatch negativity and visual working memory in school-age children with attention deficit/hyperactivity disorder.

Background: Attention-deficit/hyperactivity disorder (ADHD) patients exhibit characteristics of impaired working memory (WM) and diminished sensory processing function. This study aimed to identify the neurophysiologic basis underlying the association between visual WM and auditory processing function in children with ADHD.

Methods: The participants included 86 children with ADHD (aged 6-15 years, mean age 9.

Targeting Inflammation and Skin Aging via the Gut-Skin Axis: The Role of HY7714-Derived Extracellular Vesicles.

Intestinal mucosal tissues are prone to infections, often leading to inflammation. Lactic acid bacteria in the gut can modulate these inflammatory responses, but the interaction between host cells and lactic acid bacteria remains unclear. This study examines how HY7714 alleviates intestinal inflammation using gut-on-a-chip technology and in vitro models.

Optimization of Culture Conditions for High Cell Productivity and Astaxanthin Accumulation in Vietnam's Green Microalgae HB and a Neuroprotective Activity of Its Astaxanthin.

Background: , a green microalga, is a rich source of natural astaxanthin and a potent antioxidant with high commercial value. This study investigates the biological characteristics and potential of HB isolated from Hoa Binh, Vietnam, for growth and astaxanthin accumulation using a two-phase culture method.

Methods: HB was cultured in a C/RM medium at 25 °C, and morphological characteristics were examined.

Unveiling airborne threats: Vertical profiles of multiple emerging pollutants in PM across the urban atmosphere of Southern China.

PM has a detrimental impact on human health and has become a focus of widespread concern. The tempo-spatial distribution of emerging pollutants has been extensively studied, while there is a scarcity of understanding their vertical distribution in atmospheric environment. Here we investigated the vertical profiles of phthalate esters (PAEs), organophosphate esters (OPEs), neonicotinoids (NEOs), and per-and polyfluorinated substances (PFASs) in PM at ground level (4.