Directed Evolution of AAV9 for Efficient Gene Expression in Cardiomyocytes and .

Adeno-associated viral (AAV) vectors are increasingly used for preclinical and clinical cardiac gene therapy approaches. However, gene transfer to cardiomyocytes poses a challenge due to differences between AAV serotypes in terms of expression efficiency and . For example, AAV9 vectors work well in rodent heart muscle cells but not in cultivated neonatal rat ventricular cardiomyocytes (NRVCMs), necessitating the use of AAV6 vectors for studies.

Dynamic Measures of Team Adaptation.

Objective: Dynamic measures of team adaptation based in team cognition theory and the measurement of real-time team cognition are developed. The present study examines the validity and context-specificity of this measurement framework for simulation-based team training.

Background: Teams adapt by reorganizing their coordination behavior to overcome challenges in dynamic environments.

Self-Contamination Risk and Failure Modes During High-Level PPE Doffing: A Comparison of Two Powered Air Purifying Respirator (PAPR) Hoods: A Pilot Study.

Background: Personal protective equipment (PPE) doffing protocols can reduce risks of pathogen self-contamination. Powered air purifying respirators (PAPRs) may increase these risks. This study compares viral contamination and errors during simulated doffing of single layer vs double layer hood PAPRs.

Finerenone in Heart Failure-A Novel Therapeutic Approach.

This review will discuss heart failure, introduce a new drug finerenone, and discuss clinical studies with a focus on its effects on heart failure. Heart failure is a condition or syndrome characterized by an impairment of the pumping ability of the heart, thus no longer keeping up with the demands of the body. There are several types of heart failure; among them are heart failure with reduced ejection fraction, with mildly reduced ejection fraction and with preserved ejection fraction.

Spatial tumor immune heterogeneity facilitates subtype co-existence and therapy response in pancreatic cancer.

Pancreatic ductal adenocarcinoma (PDAC) displays a high degree of spatial subtype heterogeneity and co-existence, linked to a diverse microenvironment and worse clinical outcome. However, the underlying mechanisms remain unclear. Here, by combining preclinical models, multi-center clinical, transcriptomic, proteomic, and patient bioimaging data, we identify an interplay between neoplastic intrinsic AP1 transcription factor dichotomy and extrinsic macrophages driving subtype co-existence and an immunosuppressive microenvironment.