Simplified J774A.1 macrophage assay for fungal pathogenicity demonstrates non-clinical strains survive better than lab strains.

(formerly known as ) is the second most common cause of candidiasis, whereas the closely related yeast, causes few infections. Macrophages can control infections through phagocytosis, but in cell culture, is able to persist in macrophages better than non-pathogenic yeast. Using J774A.

Regulation of thiamine and pyruvate decarboxylase genes by Pdc2 in Nakaseomyces glabratus (Candida glabrata) is complex.

Thiamine (vitamin B1) is essential for glucose catabolism. In the yeast species, Nakaseomyces glabratus (formerly Candida glabrata) and Saccharomyces cerevisiae, the transcription factor Pdc2 (with Thi3 and Thi2) upregulates pyruvate decarboxylase (PDC) genes and thiamine biosynthetic and acquisition (THI) genes during starvation. There have not been genome-wide analyses of Pdc2 binding.

Pyruvate decarboxylase and thiamine biosynthetic genes are regulated differently by Pdc2 in S. cerevisiae and C. glabrata.

Understanding metabolism in the pathogen Candida glabrata is key to identifying new targets for antifungals. The thiamine biosynthetic (THI) pathway is partially defective in C. glabrata, but the transcription factor CgPdc2 upregulates some thiamine biosynthetic and transport genes.

Tup1 Paralog Is a Stronger Repressor of Transcription than in Candida glabrata.

is a well-characterized repressor of transcription in Saccharomyces cerevisiae and Candida albicans and is observed as a single-copy gene. We observe that most species that experienced a whole-genome duplication outside of the genus have two copies of in the yeast clade. We focused on Candida glabrata and demonstrated that the uncharacterized homolog, C.

Synthesis of Pyranopyrans Related to Diplopyrone and Evaluation as Antibacterials and Herbicides.

Stereoselective syntheses of new pyranopyrans that are related to the natural product diplopyrone, which is a phytotoxin implicated in cork oak decline, have been achieved from carbohydrate starting materials in two approaches that are based on -glycosides as key intermediates. A -alkynyl glycoside prepared by Ferrier rearrangement was used as the precursor to a new pyranopyran alkyne that showed potent antibacterial activity against the common bacterial pathogen that causes enteric septicemia in catfish. The -alkynyl glycoside also showed herbicidal activity.