Publications by authors named "D E Hacker"
Post-concussion Syndrome (PCS) describes persistent nonspecific neurological, cognitive and emotional symptoms following concussion. A young male presented to a sports concussion clinic with persistent symptoms post-injury. Neurocognitive testing found unexpected severe memory impairment.
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- AcD neurons have a unique structure where the axon comes from a basal dendrite instead of the cell body, allowing for specific action potential generation in the axon initial segment (AIS).
- Research shows that this AcD morphology develops independently of synaptic connections and the surrounding environment, starting from a single precursor neurite.
- Although the AIS in AcD neurons has a similar structure and functions like that from other neurons, it displays differences in plasticity, organelle presence, and inhibitory input, highlighting the importance of axon origin in neuron function.
Chemostat systems can be used to cultivate complex intestinal microbial communities ex vivo. Here, we present a protocol to transfer bacteria from human fecal material into chemostat systems as well as settings to simulate infant or adult colonic conditions. We describe the experimental setup, media design, donor selection, 16S rRNA amplicon sequencing, and circadian analysis of bacterial abundance.
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- Exclusive enteral nutrition (EEN) is a crucial treatment for pediatric Crohn's disease, but the exact protective mechanisms of how it works are not fully understood.
- A research study analyzed the fecal microbiota and metabolites in treatment-naive Crohn’s patients, identifying key protective features like Lachnospiraceae and medium-chain fatty acids.
- The findings demonstrated that EEN leads to distinct changes in gut microbiome compositions that vary by individual, potentially influencing the development or prevention of inflammatory bowel disease through these microbiome dynamics.
The production and purification of the secreted ectodomain of SARS-CoV-2 spike protein (S protein) were performed by transiently transfecting suspension-adapted Chinese hamster ovary cells (ExpiCHO). The method involved the separate addition of plasmid DNA expressing the S protein and polyethyleneimine to a suspension culture at a density of 5 × 10 cells/mL; and the subsequent addition of dimethyl sulfoxide at 2% (v/v). The transfected ExpiCHO cells were cultivated at 31 °C with agitation by orbital shaking under 5% CO.
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