[Trends in major tuberculosis epidemiological indices in Armenia over 10 years (1997-2006)].

The authors studied statistical data on the trend in quantitative and qualitative tuberculosis epidemiological indices in Armenia in the period of 1997 to 2006. They ascertained a 1.7-fold rise in the incidence and prevalence of tuberculosis per 100,000 (from 27.

[The effect of tularemia vaccine on some parameters of immune and non-specific anti-tumor resistance in patients with endometrial tumors].

On days 15-20 after immunization of patients suffering from endometrial tumors with tularemia live vaccine, a significant increase was registered in T-cell population of the immune system, macrophagal transformation of mononuclears and phagocyticity of blood neutrophiles which are generally reduced in stages III-IV. The study group included cases of all tumor stages. Said indices reached normal levels in stage I-II only.

[The role of terminal DNA groups in the activation of (ADP-ribose)polymerase].

Some peculiarities of activation of (ADP-ribose) polymerase by DNA fragments were studied. DNA fragments were produced by the digestion of calf thymus DNA by micrococcal nuclease and with a subsequent enzymatic modification of their end groups by nuclease S1, polynucleotide kinase of phage T4 and alkaline phosphatase. The dependence of the activating effect of DNA on the chemical structure of its end groups was established.

[Activation of (ADP-ribose) polymerase by DNA fragmented by micrococcal nuclease and DNAase I].

Activation of (ADP-ribose) polymerase by DNA fragments obtained by digestion of calf thymus DNA with micrococcal nuclease and DNAase I was studied. It was found that activation of the enzyme is due to its interaction with the terminal parts of double-stranded DNA fragments, the level of activation being independent of the size of DNA fragments.

[Reaction of poly(ADP)-ribosylation of histone H1 in the presence of P1,P4-bis(5'-adenosyl)tetraphosphate and its phosphonate analogs].

Effects of P1,P4-bis(5'-adenosyl)tetraphosphate and its phosphonate analogs on the ADP-ribosylation of H1 catalyzed by bovine testis ADP-ribose polymerase was investigated. Analogs App[CH(COCH3)]ppA and Ap[CH2]pppA as well as Ap4A inhibited poly(ADP)-ribosylation of histone H1 and at the same time accepted the ADP-ribosyl moiety of NAD. It was shown that inhibition of ADP-ribosylation of histone H1 is due to the competition of nucleotides with histone H1 for accepting ADP-ribosyl moiety of NAD on the one hand, and alteration of acceptor properties of the histone H1 on the other.