Publications by authors named "D David Collie"
Article Synopsis
- Ovine pulmonary adenocarcinoma (OPA) is a serious lung disease in sheep that affects their health and costs farmers money.* -
- Scientists created a model to study OPA by giving sheep a virus called JSRV, and they found that different amounts of the virus led to various types of tumors.* -
- They used imaging techniques like CT scans and ultrasounds to monitor the tumors, discovering that these tools can effectively track the disease's progress, which helps improve research.*
Article Synopsis
- Lung is a critical organ in radiotherapy, and damage from radiation can limit effective tumor treatment, making research into radiation-induced lung injury (RILI) important for better patient care.
- An ovine model was used to study the chronic response of lung tissue to radiation, focusing on how the damaged area differed from healthy lung over time using CT imaging and necropsy samples.
- Findings showed that irradiated lungs had reduced density and were more homogeneous compared to healthy lungs, suggesting ongoing changes in lung structure and blood vessel behavior in response to radiation exposure.
Rapid in situ detection of pathogens coupled with high resolution imaging in the distal human lung has the potential to provide new insights and diagnostic utility in patients in whom pneumonia is suspected. We have previously described an antimicrobial peptide (AMP) Ubiquicidin (fragment UBI) labelled with an environmentally sensitive fluorophore that optically detected bacteria in vitro but not ex vivo. Here, we describe further chemical development of this compound and demonstrate that altering the secondary structure of the AMP to generate a tri-branched dendrimeric scaffold provides enhanced signal in vitro and ex vivo and consequently allows the rapid detection of pathogens in situ in an explanted human lung.
View Article and Find Full Text PDFRespiratory infections in mechanically ventilated patients caused by Gram-negative bacteria are a major cause of morbidity. Rapid and unequivocal determination of the presence, localization, and abundance of bacteria is critical for positive resolution of the infections and could be used for patient stratification and for monitoring treatment efficacy. Here, we developed an in situ approach to visualize Gram-negative bacterial species and cellular infiltrates in distal human lungs in real time.
View Article and Find Full Text PDFMethods to protect against radiation-induced lung injury (RILI) will facilitate the development of more effective radio-therapeutic protocols for lung cancer and may provide the means to protect the wider population in the event of a deliberate or accidental nuclear or radiological event. We hypothesised that supplementing lipid membranes through nebulization of synthetic lamellar lipids would mitigate RILI. Following pre-treatment with either nebulised lamellar lipids or saline, anaesthetised sheep were prescribed fractionated radiotherapy (30 Gray (Gy) total dose in five 6 Gy fractions at 3-4 days intervals) to a defined unilateral lung volume.
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