π-π stacking interactions in tryptophan-lumiflavin-tyrosine: a structural model for riboflavin insertion into riboflavin-binding protein.

Context: Riboflavin (RF), also known as B2 vitamin, is the precursor to flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), two co-enzymes involved in many electron transport processes. Interactions of the isoalloxazine ring, common to all three compounds, are of great interest due to their biological function in flavoproteins and relevance in the transport by the carrier protein leading to development of drug delivery strategies and non-invasive diagnostics techniques. Based on protein crystallographic data, a computational investigation of the interactions in the complexes between lumiflavin, a model compound, and aromatic amino acids, tyrosine and tryptophan, was pursued with the goal of characterizing noncovalent interactions.

Structural Aspects of DNA Gyrase Targeted by Novel Bacterial Topoisomerase Inhibitors.

In this Letter, we present a small series of novel bacterial topoisomerase inhibitors (NTBIs) that exhibit both potent inhibition of DNA gyrase and potent antimycobacterial activity. The disclosed crystal structure of DNA gyrase in complex with DNA and compound from this NBTI series reveals the binding mode of an NBTI in the GyrA binding pocket and confirms the presence and importance of halogen bonding for the excellent on-target potency. In addition, we have shown that compound is a promising DNA gyrase inhibitor, with an IC for gyrase of 0.

How Can the Environmental Impact of Orthopaedic Surgery Be Measured and Reduced? Using Anterior Cruciate Ligament Reconstruction as a Test Case.

Background: The healthcare sector in the United States has increased its greenhouse gas emissions by 6% since 2010 and today has the highest per capita greenhouse gas emissions globally. Assessing the environmental impact and material use through the methods of life cycle assessment (LCA) and material flow analysis (MFA) of healthcare procedures, products, and processes can aid in developing impactful strategies for reductions, yet such assessments have not been performed in orthopaedic surgery. We conducted an LCA and an MFA on an ACL reconstruction (ACLR).

Perceived Health Concerns and Barriers to Care in Persons With Overweight and Obesity: A Patient Survey.

Objectives: To identify patient characteristics, health concerns, and barriers to care based on overweight or obesity.

Methods: A 50-question anonymous survey was administered to patients. Data were compared by body mass index (BMI).

Validity, Reliability, and Sensitivity of a Commercially Available Velocity Measuring Device When Performing Selected Exercises.

The aim of this study was to determine the validity, reliability, and sensitivity of a new linear position transducer (LPT) device (RepOne) to a previously validated LPT (Tendo) during the barbell back squat and bench press exercises. Fourteen recreationally resistancetrained individuals (7 males and 7 females) performed three repetitions for the back squat and bench press at loads ranging from 30-90% 1RM. Both devices recorded average (ACV) and peak (PCV) concentric velocities concurrently for every repetition at each load.